Ibrahim Basma A, Mohamed Samy H, Hassaan Mohamed M M, Sabbah Norhan A
University of Zagazig, Faculty of Medicine, Medical Biochemistry Department, Zagazig, Egypt.
University of Zagazig, Faculty of Medicine, Internal Medicine Department, Zagazig, Egypt.
J Med Biochem. 2021 Sep 3;40(4):395-406. doi: 10.5937/jomb0-30424.
Galectin-3 protein encoded by lectin galactoside-binding soluble-3 (LGALS-3) gene is an important genetic factor in type 2 diabetes mellitus (T2DM) and its cardiovascular obstacles in various populations. We aimed to elicit the pro-inflammatory effect of galectin-3 as determined by interleukin-6 (IL-6) serum levels and to explore the relationship between galectin-3 (LGALS-3 rs4652) gene variant and its expression levels with coronary artery disease (CAD) risk among T2DM Egyptian patients.
112 lean subjects were compared to 100 T2DM without CAD and 84 T2DM with CAD. A tetra-primer amplification refractory mutation system polymerase chain reaction was used to test LGALS-3 (rs4652), and galectin3 expression was tested with a quantitative real-time polymerase chain reaction. Serum IL-6 was measured using an enzyme-linked immunosorbent assay.
We found that the prevalence of LGALS-3 (rs4652) AC genotype and galectin-3 gene expression levels in T2DM with CAD were significantly higher than the additional 2 groups and were correlated positively to IL-6 circulating levels. Also, the C allele carriers (AC+CC) had significantly higher relative Galectin-3 expression levels compared to the A allele carriers (AA).
We concluded that galectin-3 expression levels and LGALS-3 (rs4652) AC genotype were coronary artery disease risk factors in people with type two diabetes among an Egyptian sample.
由凝集素半乳糖结合可溶性3（LGALS - 3）基因编码的半乳糖凝集素-3蛋白是2型糖尿病（T2DM）及其在不同人群中心血管障碍的重要遗传因素。我们旨在通过白细胞介素-6（IL - 6）血清水平确定半乳糖凝集素-3的促炎作用，并探讨半乳糖凝集素-3（LGALS - 3 rs4652）基因变异及其表达水平与埃及T2DM患者冠状动脉疾病（CAD）风险之间的关系。
将112名体型偏瘦的受试者与100名无CAD的T2DM患者和84名患有CAD的T2DM患者进行比较。采用四引物扩增阻滞突变系统聚合酶链反应检测LGALS - 3（rs4652），并通过定量实时聚合酶链反应检测半乳糖凝集素3的表达。使用酶联免疫吸附测定法测量血清IL - 6。
我们发现，患有CAD的T2DM患者中LGALS - 3（rs4652）AC基因型的患病率和半乳糖凝集素-3基因表达水平显著高于另外两组，并且与IL - 6循环水平呈正相关。此外，与A等位基因携带者（AA）相比，C等位基因携带者（AC + CC）的半乳糖凝集素-3相对表达水平显著更高。
我们得出结论，在埃及样本中，半乳糖凝集素-3表达水平和LGALS - 3（rs4652）AC基因型是2型糖尿病患者冠状动脉疾病的危险因素。
