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猕猴桃黑斑病菌的全基因组序列

Whole Genome Sequence of , the Causal Agent of Black Spot of Kiwifruit.

作者信息

Huang Ke, Tang Jianming, Zou Yong, Sun Xiangcheng, Lan Jianbin, Wang Wei, Xu Panpan, Wu Xiangwei, Ma Rui, Wang Qi, Wang Zhenshuo, Liu Jia

机构信息

College of Landscape Architecture and Life Science, Institute of Special Plants, Chongqing University of Arts and Sciences, Chongqing, China.

Institute of Microbial Ecology, Chongqing University of Arts and Sciences, Chongqing, China.

出版信息

Front Microbiol. 2021 Sep 20;12:713462. doi: 10.3389/fmicb.2021.713462. eCollection 2021.

Abstract

is a pathogen in a wide range of agriculture crops and causes significant economic losses. A strain of (Y784-BC03) was isolated and identified from "Hongyang" kiwifruit and demonstrated to cause black spot infections on fruits. The genome sequence of Y784-BC03 was obtained using Nanopore MinION technology. The assembled genome is composed of 33,869,130bp (32.30Mb) comprising 10 chromosomes and 11,954 genes. A total of 2,180 virulence factors were predicted to be present in the obtained genome sequence. The virulence factors comprised genes encoding secondary metabolites, including non-host-specific toxins, cell wall-degrading enzymes, and major transcriptional regulators. The predicted gene clusters encoding genes for the biosynthesis and export of secondary metabolites in the genome of Y784-BC03 were associated with non-host-specific toxins, including cercosporin, dothistromin, and versicolorin B. Major transcriptional regulators of different mycotoxin biosynthesis pathways were identified, including the transcriptional regulators, polyketide synthase, P450 monooxygenase, and major facilitator superfamily transporters.

摘要

是多种农作物中的一种病原体,会造成重大经济损失。从“红阳”猕猴桃中分离并鉴定出一株(Y784-BC03),它能导致果实出现黑斑感染。使用纳米孔MinION技术获得了Y784-BC03的基因组序列。组装后的基因组由33,869,130bp(32.30Mb)组成,包含10条染色体和11,954个基因。在所获得的基因组序列中预测总共存在2180个毒力因子。这些毒力因子包括编码次生代谢产物的基因,如非寄主特异性毒素、细胞壁降解酶和主要转录调节因子。预测在Y784-BC03基因组中编码次生代谢产物生物合成和输出的基因簇与非寄主特异性毒素有关,包括尾孢菌素、多斑霉素和变色菌素B。鉴定出了不同霉菌毒素生物合成途径的主要转录调节因子,包括转录调节因子、聚酮合酶、P450单加氧酶和主要易化子超家族转运蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4714/8488381/a8d6a441b22b/fmicb-12-713462-g001.jpg

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