Trigonelline Extends the Lifespan of and Delays the Progression of Age-Related Diseases by Activating AMPK, DAF-16, and HSF-1.

Trigonelline is the main alkaloid with bioactivity presented in fenugreek, which was used in traditional medicine in Asian countries for centuries. It is reported that trigonelline has anti-inflammatory, anti-oxidant, and anti-pathogenic effects. We are wondering whether trigonelline have anti-aging effect. We found that 50 M of trigonelline had the best anti-aging activity and could prolong the lifespan of () by about 17.9%. Trigonelline can enhance the oxidative, heat, and pathogenic stress resistance of . Trigonelline could also delay the development of neurodegenerative diseases, such as AD, PD, and HD, in models of . Trigonelline could not prolong the lifespan of long-lived worms with loss-of-function mutations in genes regulating energy and nutrition, such as , , , and . Trigonelline requires , , and to extend the lifespan of . Trigonelline can also up-regulate the expression of and targeted downstream genes, such as , , , and . Our results can be the basis for developing trigonelline-rich products with health benefits, as well as for further research on the pharmacological usage of trigonelline.