Hernández-Morales Miriam, Han Victor, Kramer Richard H, Liu Chunlei
Department of Electrical Engineering and Computer Sciences, University of California, 505 Cory Hall MC# 1770, Berkeley, CA 94720, USA.
Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, USA.
iScience. 2021 Sep 9;24(10):103094. doi: 10.1016/j.isci.2021.103094. eCollection 2021 Oct 22.
FeRIC (Ferritin iron Redistribution to Ion Channels) is a magnetogenetic technique that uses radiofrequency (RF) alternating magnetic fields to activate the transient receptor potential channels, TRPV1 and TRPV4, coupled to cellular ferritins. In cells expressing ferritin-tagged TRPV, RF stimulation increases the cytosolic Ca levels via a biochemical pathway. The interaction between RF and ferritin increases the free cytosolic iron levels that, in turn, trigger chemical reactions producing reactive oxygen species and oxidized lipids that activate the ferritin-tagged TRPV. In this pathway, it is expected that experimental factors that disturb the ferritin expression, the ferritin iron load, the TRPV functional expression, or the cellular redox state will impact the efficiency of RF in activating ferritin-tagged TRPV. Here, we examined several experimental factors that either enhance or abolish the RF control of ferritin-tagged TRPV. The findings may help optimize and establish reproducible magnetogenetic protocols.
铁蛋白铁重新分布至离子通道（FeRIC）是一种磁遗传技术，它利用射频（RF）交变磁场来激活与细胞铁蛋白偶联的瞬时受体电位通道TRPV1和TRPV4。在表达铁蛋白标记TRPV的细胞中，RF刺激通过生化途径增加胞质钙水平。RF与铁蛋白之间的相互作用增加了胞质游离铁水平，进而引发化学反应，产生活性氧和氧化脂质，从而激活铁蛋白标记的TRPV。在这条途径中，可以预期，干扰铁蛋白表达、铁蛋白铁负载、TRPV功能表达或细胞氧化还原状态的实验因素将影响RF激活铁蛋白标记TRPV的效率。在此，我们研究了几种增强或消除RF对铁蛋白标记TRPV控制的实验因素。这些发现可能有助于优化并建立可重复的磁遗传方案。
