Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, China.
Int J Radiat Biol. 2022;98(1):11-17. doi: 10.1080/09553002.2021.1987561. Epub 2021 Oct 12.
Emerging evidence has shown that radiotherapy is an effective treatment for hepatocellular carcinoma (HCC), Micro(mi)RNAs are involved in regulating radiosensitivity in many cancers. MiR-122 accounts for approximately 70% of all cloned miRNAs in the liver, but there are few reports about whether it is involved in regulating of radiosensitivity in HCC cells.
HCC cells (HepG2 and Huh7) overexpressing miR-122 were constructed by transfecting them with lentiviral-miR-122. Then, their proliferation ability was analyzed by the MTT, and colony formation assays and a xenograft tumor model was used to detect their radiosensitivity. The expression of cyclin G1 mRNA and protein was detected by the quantitative real-time polymerase chain reaction and western blotting, respectively.
Overexpression of miR-122 inhibited the proliferation of, and radiosensitized HCC cells. Cyclin G1 mRNA and protein level were suppressed in HepG2 tumors overexpression miR-122.
MiR-122 may be useful as a potential radiosensitizer for HCC, and its mechanism is related to the regulation of cyclin G1.
新出现的证据表明，放射治疗是治疗肝细胞癌（HCC）的有效方法。Micro(mi)RNAs 参与调节许多癌症的放射敏感性。miR-122 约占肝脏中所有克隆 miRNAs 的 70%，但关于它是否参与调节 HCC 细胞的放射敏感性的报道很少。
通过慢病毒转染构建 miR-122 过表达的 HCC 细胞（HepG2 和 Huh7）。然后，通过 MTT、集落形成实验和异种移植肿瘤模型分析它们的增殖能力，检测它们的放射敏感性。通过实时定量聚合酶链反应和 Western blot 分别检测细胞周期蛋白 G1 mRNA 和蛋白的表达。
miR-122 的过表达抑制了 HCC 细胞的增殖并放射增敏。miR-122 过表达的 HepG2 肿瘤中细胞周期蛋白 G1 mRNA 和蛋白水平受到抑制。
miR-122 可能作为 HCC 的潜在放射增敏剂有用，其机制与 cyclin G1 的调节有关。
