Department of Microbiology, Faculty of Science, Chulalongkorn University, 254 Phyathai Road, Pathumwan, Bangkok, 10330, Thailand; Microbial Technology for Marine Pollution Treatment Research Unit, Department of Microbiology, Faculty of Science, Chulalongkorn University, 254 Phyathai Road, Pathumwan, Bangkok, 10330, Thailand.
Microbial Technology for Marine Pollution Treatment Research Unit, Department of Microbiology, Faculty of Science, Chulalongkorn University, 254 Phyathai Road, Pathumwan, Bangkok, 10330, Thailand.
Environ Pollut. 2022 Jan 1;292(Pt A):118309. doi: 10.1016/j.envpol.2021.118309. Epub 2021 Oct 6.
A pyrene-degrading consortium OPK containing Mycolicibacterium strains PO1 and PO2, Novosphingobium pentaromativorans PY1 and Bacillus subtilis FW1 effectively biodegraded medium- and long-chain alkanes as well as mixed hydrocarbons in crude oil. The detection of alkB and CYP153 genes in the genome of OPK members supports its phenotypic ability to effectively degrade a broad range of saturated hydrocarbons in crude oil. Zeolite-immobilized OPK was developed as a ready-to-use bioproduct and it exhibited 74% removal of 1000 mg L crude oil within 96 h in sterilized seawater without nutrient supplementation and maintained high crude oil-removal activity under a broad range of pH values (5.0-9.0), temperatures (30-40 °C) and salinities (20-60‰). In addition, the immobilized OPK retained a high crude oil removal efficacy in semicontinuous experiments and showed reusability for at least 5 cycles. Remarkably, bioaugmentation with zeolite-immobilized OPK in sandy soil microcosms significantly increased crude oil (10,000 mg kg soil) removal from 45% to 80.67% within 21 days compared to biostimulation and natural attenuation. Moreover, bioaugmentation with exogenous immobilized OPK stimulated an increase in the relative abundances of Alcanivorax genus, indigenous hydrocarbon-degrading bacteria, which in turn enhanced removal efficiency of crude oil contamination from sandy soil microcosms. The results indicate positive interactions between the bioaugmented immobilized consortium, harboring Mycolicibacterium as a key player, and indigenous Alcanivorax, which exhibited crucial functions for improving crude oil removal efficacy. The knowledge obtained forms an important basis for further synthesis and handling of a promising bio-based product for enhancing the in situ bioremediation of crude oil-polluted marine environments.
OPK 是一个包含分枝杆菌菌株 PO1 和 PO2、新鞘氨醇单胞菌 PY1 和枯草芽孢杆菌 FW1 的芘降解菌联合体，可有效生物降解中链和长链烷烃以及原油中的混合烃。在 OPK 成员的基因组中检测到 alkB 和 CYP153 基因，支持其表型能力，可有效降解原油中广泛的饱和烃。沸石固定化 OPK 被开发为即用型生物产品，在没有营养补充的情况下，在无菌海水中 96 小时内可去除 1000mg/L 原油的 74%，并在广泛的 pH 值（5.0-9.0）、温度（30-40°C）和盐度（20-60‰）下保持高的原油去除活性。此外，固定化 OPK 在半连续实验中保留了高的原油去除效果，并且至少可重复使用 5 次。值得注意的是，在沙土微宇宙中，沸石固定化 OPK 的生物强化可将原油（10000mgkg 土壤）的去除率从 45%显著提高到 80.67%，而生物刺激和自然衰减的去除率为 45%。此外，外源固定化 OPK 的生物强化刺激了土著烃降解菌中 Alcanivorax 属的相对丰度增加，从而提高了沙土微宇宙中原油污染的去除效率。结果表明，生物强化固定联合体与土著 Alcanivorax 之间存在积极的相互作用，作为关键参与者的分枝杆菌，以及表现出提高原油去除效率的关键功能的土著 Alcanivorax。该研究结果为进一步合成和处理有前途的生物基产品提供了重要基础，以增强受原油污染的海洋环境的原位生物修复。
