Mehboob Madeeha, Rehman Abdul, Naz Iffat, Shuja Malik Nawaz, Farooq Ayesha Shuja, Khattak Baharullah
Department of Microbiology, Kohat University of Science and Technology (KUST), Kohat 26000, Khyber Pakhtunkhwa, Pakistan.
Department of Biology, College of Science, Qassim University, Buraydah 51452, Saudi Arabia.
ACS Omega. 2025 Jan 16;10(3):2501-2516. doi: 10.1021/acsomega.4c06434. eCollection 2025 Jan 28.
This study isolates oil-degrading bacteria from contaminated soil in automobile workshops, aiming to address environmental pollution from petroleum spills. Nineteen bacterial isolates were screened, with three isolates S4 (), M8 (), and M9 (), showing significant oil degradation potency. In shake flask assays, S4 degraded 57.8%, M8 60.2%, and M9 58.1% of oil at a 5% concentration. Molecular identification through 16S rRNA gene sequencing confirmed these strains. Plate assays also supported the degradation potential, with growth zones of 38.4 ± 1.3 mm (S4), 39.7 ± 1.9 mm (M8), and 41.2 ± 1.7 mm (M9). Gas chromatography-mass spectrometry analysis of treated oil indicated the presence of hydrocarbons, phenolic compounds, and esters, suggesting microbial interactions, potentially involving the breakdown or transformation of complex hydrocarbons into simpler compounds by bacterial enzymes. These results highlight the potential of these bacterial strains, particularly S4, for the bioremediation of petroleum-contaminated sites.
本研究从汽车维修车间受污染的土壤中分离出石油降解菌，旨在解决石油泄漏造成的环境污染问题。筛选出了19株细菌分离株，其中三株分离株S4（）、M8（）和M9（）表现出显著的石油降解能力。在摇瓶试验中，S4在5%浓度下可降解57.8%的石油，M8为60.2%，M9为58.1%。通过16S rRNA基因测序进行的分子鉴定证实了这些菌株。平板试验也支持了其降解潜力，S4的生长区为38.4±1.3毫米，M8为39.7±1.9毫米，M9为41.2±1.7毫米。对处理后的石油进行气相色谱-质谱分析表明存在碳氢化合物、酚类化合物和酯类，这表明存在微生物相互作用，可能涉及细菌酶将复杂碳氢化合物分解或转化为更简单的化合物。这些结果突出了这些细菌菌株，特别是S4，在石油污染场地生物修复方面的潜力。
