Shao Ming, Yang Qian-Kun, Zhu Wei-Tao, Kong Yong-Kui, Song Jie, Wang Jing, Lyu Xian-Ping
Department of Blood Transfusion,The First Affiliated Hospital of Zhengzhou University , Zhengzhou, 450052, Henan Province, China.
Department of Laboratory Medicine, The First Affiliated Hospital of Zhengzhou University , Zhengzhou, 450052, Henan Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2021 Oct;29(5):1589-1594. doi: 10.19746/j.cnki.issn.1009-2137.2021.05.034.
To detect the ABO / RhD blood type of infants younger than 6 months in different gestational age and month old with automatic microcolumn glass sphere and tube method, and compare the result of the two methods.
The data of 896 samples of infants younger than 6 months from January 2018 to February 2019 was collected. The two methods were used to detect ABO/RhD blood type in all samples and compare the detection rate of ABO/RhD antigen and ABO reverse typing and agglutination intensity of the two methods.
Three hundred and eight cases of type A (34.4%), 281 cases of type B (31.4%), 210 cases of type O (23.4%), 97 cases of type AB (10.8%), and 896 positive cases of RhD blood type were detected out by two methods. There were no significant differences of ABO/RhD antigen agglutination intensity between two methods (P > 0.05). Except for type AB, the detection rate of ABO reverse typing in infants with type B was significantly higher than that with type A and type O (P < 0.05). The agglutination intensity of type A reverse cell was higher than type B reverse cell (P < 0.05). The fully automatic microcolumn glass sphere method exhibited higher detection rate of ABO reverse typing in the samples of type A and type O group and agglutination intensity of ABO reverse typing in all types as compared with the tube method (P < 0.05). The detection rate and agglutination intensity of ABO reverse typing in term group were significantly higher than those in preterm group (P < 0.05). The fully automatic microcolumn glass sphere method exhibited higher detection rate of ABO reverse typing and agglutination intensity compared with the tube method between two groups (P < 0.05). The detection rate and agglutination intensity of ABO reverse typing in group IV (4-6 months old) were significantly higher than those in groups I, II and III (young than 3 months old) (P < 0.05). The fully automatic microcolumn glass sphere method exhibited higher detection rate of ABO reverse typing in I, II, III groups and agglutination intensity of ABO reverse typing in the 4 groups compared with the tube method (P < 0.05).
ABO / RhD blood group antigen can be accurated detected in majority of infants, but the detection rate of ABO antibody is related to gestational age and month age of infants. The detection rate and agglutination intensity of the fully automatic microcolumn glass sphere method in ABO reverse typing are higher than those of the tube method, especially for premature infants and children within 3 months old.
采用自动微柱玻璃珠法和试管法检测不同胎龄和月龄的6个月以下婴儿的ABO/RhD血型，并比较两种方法的检测结果。
收集2018年1月至2019年2月896例6个月以下婴儿的样本数据。用两种方法检测所有样本的ABO/RhD血型，比较两种方法ABO/RhD抗原检测率、ABO反定型及凝集强度。
两种方法共检测出A型308例（34.4%）、B型281例（31.4%）、O型210例（23.4%）、AB型97例（10.8%），RhD血型阳性896例。两种方法ABO/RhD抗原凝集强度差异无统计学意义（P>0.05）。除AB型外，B型婴儿ABO反定型检测率显著高于A型和O型婴儿（P<0.05）。A型反定型细胞凝集强度高于B型反定型细胞（P<0.05）。全自动微柱玻璃珠法在A型和O型组样本中的ABO反定型检测率及各血型ABO反定型凝集强度均高于试管法（P<0.05）。足月儿组ABO反定型检测率和凝集强度显著高于早产儿组（P<0.05）。两组间全自动微柱玻璃珠法ABO反定型检测率和凝集强度均高于试管法（P<0.05）。IV组（4-6个月龄）ABO反定型检测率和凝集强度显著高于I组、II组和III组（小于3个月龄）（P<0.05）。全自动微柱玻璃珠法在I、II、III组的ABO反定型检测率及4组的ABO反定型凝集强度均高于试管法（P<0.05）。
多数6个月以下婴儿可准确检测ABO/RhD血型，但ABO抗体检测率与婴儿胎龄和月龄有关。全自动微柱玻璃珠法ABO反定型检测率和凝集强度高于试管法，尤其对于早产儿和3个月以内的婴儿。
