Department of Stomatology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, China.
Department of Stomatology, Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.
Ann Palliat Med. 2021 Sep;10(9):9685-9691. doi: 10.21037/apm-21-2277.
It is well known that diabetes mellitus is one of the high-risk factors for periodontitis and also for the failure of implant restorations. Usually, the success of an implant restoration depends on both the good osseointegration and the stable soft tissue interface on the implant neck. A good gingival interface of the implant neck is the barrier that enables implant to resist oral microorganisms and the site of initiation of peri-implantitis. This study sought to investigate the effects of hyperglycemia on the attachment and proliferation of human gingival fibroblasts (HGFs) on pure titanium surfaces.
HGFs were cultured in cell culture mediums with different glucose concentrations (i.e., 5.5, 8. 8, 10, and 15 mmol/L) for 7 d and seeded on pure titanium surfaces. The cells that were seeded on the titanium surfaces had been cultured in cell culture mediums with different glucose concentrations for 3 and 7 d. The attached HGFs on the titanium surfaces were counted for all groups using a blood cell counting plate, and the results were statistically analyzed. The morphologies of the attached HGFs on the titanium surfaces were observed for all the groups using a scanning electronic microscope.
As the glucose concentrations increased, the number of attached HGFs on the titanium surfaces decreased. The numbers of attached cells in Groups A and B 7 d after being seeded on the titanium surfaces were more than those 3 d after being seeded (P<0.05). The numbers of attached cells in Groups C and D 3 d after being seeded on the titanium were more than those 7 d after being seeded (P<0.05). The scanning electronic microscope showed that the attached cells in Groups A and B proliferated well, and most cells grew one on top of another. Conversely, the attached cells in Groups C and D proliferated sparsely and the cell morphologies were not good.
The attachment and proliferation of HGFs on pure titanium surfaces were inhibited by increases in glucose concentrations, and the inhibition was further enhanced by the passage of time.
众所周知，糖尿病是牙周炎和种植体修复失败的高危因素之一。通常，种植体修复的成功取决于良好的骨整合和种植体颈部稳定的软组织界面。种植体颈部良好的牙龈界面是使种植体抵抗口腔微生物和启动种植体周围炎的屏障。本研究旨在探讨高血糖对人牙龈成纤维细胞（HGFs）在纯钛表面黏附及增殖的影响。
将 HGFs 分别培养于含不同浓度葡萄糖（5.5、8.8、10 和 15mmol/L）的细胞培养液中 7d，然后接种于纯钛表面。将不同浓度葡萄糖培养液中培养 3 和 7d 的细胞接种于钛表面。采用血球计数板对各组钛表面黏附的 HGFs 进行计数，并进行统计学分析。采用扫描电子显微镜观察各组钛表面黏附的 HGFs 形态。
随着葡萄糖浓度的增加，钛表面黏附的 HGFs 数量减少。钛表面接种 7d 后 A、B 组的黏附细胞数多于接种 3d 后（P<0.05）；钛表面接种 3d 后 C、D 组的黏附细胞数多于接种 7d 后（P<0.05）。扫描电镜显示 A、B 组黏附细胞增殖良好，多数细胞相互重叠生长；而 C、D 组黏附细胞增殖稀疏，细胞形态不佳。
随着葡萄糖浓度的增加，HGFs 在纯钛表面的黏附和增殖受到抑制，且随着时间的推移，抑制作用进一步增强。
