Bakitian Fahad, Alshammari Hatem, Youssef Abdel-Rahman, Hawsawi Rayan A, Alreshedi Anwar Doufan, Alkhashram Mariam Abdulaziz
Department of Restorative Dentistry, Faculty of Dentistry, Umm Al-Qura University, Makkah, Saudi Arabia.
Department of Preventive Dentistry, College of Dentistry, University of Hail, Hail, Saudi Arabia.
Clin Cosmet Investig Dent. 2024 May 13;16:127-134. doi: 10.2147/CCIDE.S462763. eCollection 2024.
Applying multifunctional coatings employing strontium (Sr) ions on titanium (Ti) surfaces is a useful and biocompatible method to improve osseointegration and prevent tissue infections through antimicrobial activity. Nonetheless, the effectiveness of Sr coating on the adhesion and viability of human gingival fibroblasts (HGFs) to Ti surfaces remains unclear.
The study aimed to evaluate the effect of Sr coating on the adhesion and viability of HGFs to Ti surfaces.
The Ti wafers were divided into two groups based on Sr coating: uncoated Ti (control) and Sr-coated Ti. The Magnetron sputtering technique was used for Sr coating on Ti surfaces. The HGFs were seeded onto the surfaces and cultured for 48 and 96 hours before the cell adhesion and viability of the attached HGFs were assessed. The adhesion of HGFs was analyzed using the attached cell numbers at 48 h and 96 h, and the morphology at 24 h and 72 h. The cytotoxic effect on HGFs was assessed after 24 and 72 hours of incubation using cell viability assay. Student's -test was used for statistical analysis.
The number of cells attached to Sr-coated surfaces was significantly greater than those attached to uncoated Ti surfaces after 48 hours (P<0.0001) and 96 hours (P=0.0002). Sr-coated and uncoated Ti surfaces were not cytotoxic to HGFs, with the cell viability ranging from 92% to 105% of the untreated control HGFs. There were no significant differences in cell viability between Sr-coated and uncoated Ti surfaces at 24 hours (P=0.3675) and 72 hours (P=0.0982).
Sr-coated Ti surfaces induce adhesion of HGFs compared to uncoated Ti surfaces. Further, Sr-coated and uncoated Ti surfaces show no cytotoxic effect on the attached HGFs.
在钛（Ti）表面应用含锶（Sr）离子的多功能涂层是一种有用的生物相容性方法，可通过抗菌活性改善骨整合并预防组织感染。然而，Sr涂层对人牙龈成纤维细胞（HGFs）在Ti表面的黏附及活力的影响仍不明确。
本研究旨在评估Sr涂层对HGFs在Ti表面黏附及活力的影响。
根据Sr涂层将Ti片分为两组：未涂层Ti（对照组）和Sr涂层Ti。采用磁控溅射技术在Ti表面进行Sr涂层。将HGFs接种到表面并培养48小时和96小时，然后评估附着的HGFs的细胞黏附及活力。通过48小时和96小时时的附着细胞数量以及24小时和72小时时的形态分析HGFs的黏附情况。使用细胞活力测定法在孵育24小时和72小时后评估对HGFs的细胞毒性作用。采用学生t检验进行统计分析。
48小时（P<0.0001）和96小时（P=0.0
