Lin Jhih-Ni, Chi Chih-Ying, Lin Yu-Ying, Kuan Che-Yung, Chang Chia-Tien, Lin Li-Ze, Yang I-Hsuan, Lin Feng-Huei
Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, 106319, Taiwan.
Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli County, Zhunan, 350401, Taiwan.
J Biol Eng. 2025 Apr 28;19(1):39. doi: 10.1186/s13036-025-00505-z.
Excessive caloric intake and reduced energy expenditure contribute to obesity and localized fat accumulation, adversely affecting overall health. Despite advancements in obesity treatments, noninvasive approaches for targeted fat reduction remain limited. This study introduces a novel sonosensitizer microparticle, cerium-doped calcium carbonate (CaCO:Ce), and evaluates its potential application in combination with low-intensity ultrasound (LIUS) for noninvasive body sculpting. CaCO:Ce was synthesized via an environmentally friendly method, producing uniform 1.77 μm particles optimized for endocytosis. Energy-dispersive X-ray and X-ray photoelectron spectroscopy confirmed successful cerium doping. The particles demonstrated excellent biocompatibility and generated reactive oxygen species under LIUS exposure. Safety was validated through biochemical, hematological, and histological analyses in Sprague-Dawley rats. Animal experiments further revealed that CaCO:Ce combined with ultrasound significantly reduced body weight growth rates, waistline measurements, and subcutaneous fat accumulation. These findings suggest that CaCO:Ce, coupled with LIUS, offers a promising, noninvasive, and low-risk strategy for body sculpting, addressing limitations of current methodologies.
热量摄入过多和能量消耗减少会导致肥胖和局部脂肪堆积，对整体健康产生不利影响。尽管肥胖治疗取得了进展，但用于有针对性的减脂的非侵入性方法仍然有限。本研究引入了一种新型声敏剂微粒，即铈掺杂碳酸钙（CaCO:Ce），并评估其与低强度超声（LIUS）联合用于非侵入性身体塑形的潜在应用。CaCO:Ce是通过一种环保方法合成的，产生了均匀的1.77μm颗粒，这些颗粒针对内吞作用进行了优化。能量色散X射线和X射线光电子能谱证实了铈的成功掺杂。这些颗粒表现出优异的生物相容性，并在LIUS照射下产生活性氧。通过对Sprague-Dawley大鼠进行生化、血液学和组织学分析验证了安全性。动物实验进一步表明，CaCO:Ce与超声联合使用可显著降低体重增长率、腰围测量值和皮下脂肪堆积。这些发现表明，CaCO:Ce与LIUS相结合，为身体塑形提供了一种有前景、非侵入性且低风险的策略，解决了当前方法的局限性。
