van Besouw Nicole M, Rojas Aleixandra Mendoza, See Sarah B, de Kuiper Ronella, Dieterich Marjolein, Roelen Dave L, Clahsen-van Groningen Marian C, Hesselink Dennis A, Zorn Emmanuel, Baan Carla C
Erasmus MC Transplantation Institute, Department of Internal Medicine-Nephrology & Transplantation and Pathology, University Medical Center Rotterdam, Rotterdam, Netherlands.
Columbia Center for Translational Immunology, Columbia University Medical Irving Center, New York, USA.
J Transplant. 2021 Sep 30;2021:7005080. doi: 10.1155/2021/7005080. eCollection 2021.
The relationship between circulating effector memory T and B cells long after transplantation and their susceptibility to immunosuppression are unknown. To investigate the impact of antirejection therapy on T cell-B cell coordinated immune responses, we assessed IFN--producing memory cells and natural antibodies (nAbs) that potentially bind to autoantigens on the graft.
Plasma levels of IgG nAbs to malondialdehyde (MDA) were measured in 145 kidney transplant recipients at 5-7 years after transplantation. In 54 of these patients, the number of donor-reactive IFN--producing cells was determined. 35/145 patients experienced rejection, 18 of which occurred within 1 year after transplantation.
The number of donor-reactive IFN--producing cells and the levels of nAbs were comparable between rejectors and nonrejectors. The nAbs levels were positively correlated with the number of donor-reactive IFN--producing cells ( = 0.39, =0.004). The positive correlation was only observed in rejectors ( = 0.53, =0.003; nonrejectors: = 0.24, =0.23). Moreover, we observed that intravenous immune globulin treatment affected the level of nAbs and this effect was found in patients who experienced a late ca-ABMR compared to nonrejectors (=0.008).
The positive correlation found between alloreactive T cells and nAbs in rejectors suggests an intricate role for both components of the immune response in the rejection process. Treatment with intravenous immune globulin impacted nAbs.
移植后很长时间循环效应记忆T细胞和B细胞之间的关系及其对免疫抑制的易感性尚不清楚。为了研究抗排斥治疗对T细胞 - B细胞协调免疫反应的影响，我们评估了产生干扰素 - γ的记忆细胞和可能与移植物自身抗原结合的天然抗体（nAbs）。
在145名肾移植受者移植后5 - 7年测量其血浆中针对丙二醛（MDA）的IgG nAbs水平。在其中54名患者中，测定了供体反应性产生干扰素 - γ的细胞数量。145名患者中有35名发生排斥反应，其中18名发生在移植后1年内。
排斥反应者和非排斥反应者之间供体反应性产生干扰素 - γ的细胞数量和nAbs水平相当。nAbs水平与供体反应性产生干扰素 - γ的细胞数量呈正相关（r = 0.39，P = 0.004）。这种正相关仅在排斥反应者中观察到（r = 0.53，P = 0.003；非排斥反应者：r = 0.24，P = 0.23）。此外，我们观察到静脉注射免疫球蛋白治疗会影响nAbs水平，并且在发生晚期钙调神经磷酸酶抑制剂介导的抗体介导排斥反应（ca - ABMR）的患者中比非排斥反应者更明显（P = 0.008）。
排斥反应者中同种异体反应性T细胞与nAbs之间的正相关表明免疫反应的这两个组成部分在排斥过程中发挥着复杂的作用。静脉注射免疫球蛋白治疗会影响nAbs。
