RNA干扰介导的尖孢镰刀菌番茄专化型中某些基因的沉默赋予番茄对枯萎病的抗性。
RNAi-mediated silencing of and enes of f. sp. confers resistance against Fusarium wilt in tomato.
作者信息
Tetorya Meenakshi, Rajam Manchikatla Venkat
机构信息
Department of Genetics, University of Delhi-South Campus, Benito Juarez Marg, New Delhi, 110021 India.
出版信息
3 Biotech. 2021 Oct;11(10):443. doi: 10.1007/s13205-021-02973-8. Epub 2021 Sep 21.
UNLABELLED
In the present study, we have explored the potential of the RNAi mediated silencing of genes encoding peroxisomal biogenesis factor and β-1,3-glucanosyltransferase in f. sp. lyco to confer resistance to Fusarium wilt in transgenic tomato plants. The partial gene fragments from these genes were utilized independently to generate hairpin RNAi constructs in appropriate silencing vectors and used for -mediated transformation of tomato. The presence of gene-specific siRNAs was confirmed by stem-loop RT-PCR analysis of selected transgenic tomato lines. Transgenic lines expressing gene-specific dsRNA displayed enhanced resistance to with delayed development of disease symptoms. The survival rate of transgenic tomato lines after fungal infection was higher as compared to that of the untransformed tomato plants.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s13205-021-02973-8.
未标记
在本研究中，我们探索了RNA干扰介导的沉默编码过氧化物酶体生物发生因子和β-1,3-葡聚糖基转移酶的基因在番茄枯萎病菌中的潜力，以赋予转基因番茄植株对枯萎病的抗性。这些基因的部分基因片段被独立用于在合适的沉默载体中构建发夹RNA干扰结构，并用于番茄的介导转化。通过对选定转基因番茄品系的茎环RT-PCR分析证实了基因特异性小干扰RNA的存在。表达基因特异性双链RNA的转基因品系对番茄枯萎病菌表现出增强的抗性，且病害症状发展延迟。与未转化的番茄植株相比，真菌感染后转基因番茄品系的存活率更高。
补充信息
在线版本包含可在10.1007/s13205-021-02973-8获取的补充材料。
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