Zhai Shao-Lun, Xie Yi-Lun, Zhai Qi, Wen Xiao-Hui, Lv Dian-Hong, Chen Qin-Ling, Wang Gang, Wei Wen-Kang
Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture of Rural Affairs, and Key Laboratory of Animal Disease Prevention of Guangdong Province, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Agro-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Front Vet Sci. 2021 Sep 22;8:721284. doi: 10.3389/fvets.2021.721284. eCollection 2021.
Bovine rhinitis B virus (BRBV) is an emerging viral species in the genus , family Picornaviridae. Studies suggested that BRBV was considered a potential etiological agent of bovine respiratory disease complex (BRDC). BRBV has been reported in the United States, Sweden, Canada, Japan, and Mexico. However, little information of BRBV was available in China. In this study, we performed viral metagenomic analysis in a calf with respiratory disease. The results showed high abundance (3.85) of BRBV nucleotide and 248 mapped reads in calf samples. Online BLASTn analysis showed that three contigs of those had the highest nucleotide similarity (95%) with one Swedish BRBV isolate (BRBV_SWE1, GenBank accession no. KY432299). To identify the genome characterization of the Chinese BRBV isolate (designated CHN1), six couples of overlapping RT-PCR primers were designed according to genome sequences of BRBV_SWE1. Through gene cloning and splicing, we obtained the genome information of CHN1, possessing 7,465 nucleotides (46.6% G+C). Although CHN1 had the highest nucleotide similarity (95.1%) with BRBV_SWE1, one 11-nucleotide (ACATTTGTTGT) deletion occurred in the 5' untranslated region compared to SWE1. Phylogenetic analysis showed that CHN1 clustered together with BRBV_SWE1, and far from other BRBV isolates. This study recorded the first discovery of BRBV infection in China. Further investigation should be made in order to evaluate the infection status and epidemiological significance of BRBV in China.
牛鼻炎B病毒（BRBV）是小RNA病毒科属中的一种新兴病毒。研究表明，BRBV被认为是牛呼吸道疾病综合征（BRDC）的潜在病原体。在美国、瑞典、加拿大、日本和墨西哥均有BRBV的报道。然而，中国关于BRBV的信息较少。在本研究中，我们对一头患有呼吸道疾病的小牛进行了病毒宏基因组分析。结果显示，小牛样本中BRBV核苷酸丰度较高（3.85），有248条比对 reads。在线BLASTn分析表明，其中三个重叠群与一株瑞典BRBV分离株（BRBV_SWE1，GenBank登录号KY432299）具有最高的核苷酸相似性（95%）。为了鉴定中国BRBV分离株（命名为CHN1）的基因组特征，根据BRBV_SWE1的基因组序列设计了六对重叠RT-PCR引物。通过基因克隆和拼接，我们获得了CHN1的基因组信息，其长度为7465个核苷酸（G+C含量为46.6%）。尽管CHN1与BRBV_SWE1具有最高的核苷酸相似性（95.1%），但与SWE1相比，其5'非翻译区发生了一个11个核苷酸（ACATTTGTTGT）的缺失。系统发育分析表明，CHN1与BRBV_SWE1聚类在一起，与其他BRBV分离株距离较远。本研究记录了中国首次发现BRBV感染。为评估BRBV在中国的感染状况和流行病学意义，应进一步开展调查。
