通过酶联免疫吸附测定法对拟杆菌属进行血清学鉴定。
Serological identification of Bacteroides species by an enzyme-linked immunosorbent assay.
作者信息
Poxton I R
出版信息
J Clin Pathol. 1979 Mar;32(3):294-8. doi: 10.1136/jcp.32.3.294.
Abstract
An enzyme-linked immunosorbent assay was used to titrate antisera raised against live cultures of eight type species (biotypes) of Bacteroides with the EDTA-released outer-membrane complex from 29 characterised strains of Bacteroides species. With only minor exceptions, the strains investigated reacted to titre with the antisera raised against the homologous type species and not against the heterologous type species. Cross-reactivity between heterologous species and antiserum was only significant between closely related biotypes. This cross-reactivity could be removed by absorption of the antisera with whole cells. Significant correlation was found between serotype and biotype with this technique.
摘要
采用酶联免疫吸附测定法，用来自29株已鉴定的拟杆菌属菌株的经乙二胺四乙酸（EDTA）释放的外膜复合物，对针对8种拟杆菌属模式种（生物型）活培养物产生的抗血清进行滴定。除了少数例外情况，所研究的菌株与针对同源模式种产生的抗血清反应至滴定度，而不与异源模式种产生的抗血清反应。异源种与抗血清之间的交叉反应仅在密切相关的生物型之间显著。这种交叉反应可以通过用全细胞吸收抗血清来消除。用该技术发现血清型与生物型之间存在显著相关性。
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