通过酶联免疫吸附测定法对口腔拟杆菌属进行血清学鉴定。
Serological identification of oral Bacteroides spp. by enzyme-linked immunosorbent assay.
作者信息
Ebersole J L, Frey D E, Taubman M A, Smith D J, Socransky S S, Tanner A C
出版信息
J Clin Microbiol. 1984 May;19(5):639-44. doi: 10.1128/jcm.19.5.639-644.1984.
Abstract
A rapid method for identifying black-pigmented oral Bacteroides spp. is described. Species-specific rabbit antisera to Bacteroides gingivalis, B. intermedius, and B. melaninogenicus were used in an enzyme-linked immunosorbent assay to identify clinical isolates of black-pigmented Bacteroides spp. from humans. The results showed excellent agreement with biochemical identification of B. gingivalis and B. intermedius. Only 36% of the B. melaninogenicus isolates were identified with the enzyme-linked immunosorbent assay, suggesting that this group of black-pigmented Bacteroides spp. is made up of more than one serotype. The serological enzyme-linked immunosorbent assay should enable rapid identification of black-pigmented Bacteroides spp. isolated from sites of oral diseases and may also be used to identify the presence of these organisms in complex bacterial mixtures from oral sites.
摘要
本文描述了一种快速鉴定产黑色素口腔拟杆菌属菌种的方法。使用针对牙龈拟杆菌、中间拟杆菌和产黑色素拟杆菌的种特异性兔抗血清，通过酶联免疫吸附测定法来鉴定从人类分离出的产黑色素拟杆菌属菌种的临床分离株。结果显示，该方法与牙龈拟杆菌和中间拟杆菌的生化鉴定结果高度一致。通过酶联免疫吸附测定法仅鉴定出36%的产黑色素拟杆菌分离株，这表明这一组产黑色素拟杆菌属菌种由不止一种血清型组成。血清学酶联免疫吸附测定法应能够快速鉴定从口腔疾病部位分离出的产黑色素拟杆菌属菌种，也可用于鉴定口腔部位复杂细菌混合物中这些微生物的存在情况。
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