Borel Patrick, Troadec Romane, Damiani Morgane, Halimi Charlotte, Nowicki Marion, Guichard Philippe, Margier Marielle, Astier Julien, Grino Michel, Reboul Emmanuelle, Landrier Jean-François
C2VN, INRAE, INSERM, Aix-Marseille Univ, Marseille, France.
Mol Nutr Food Res. 2021 Nov;65(22):e2100650. doi: 10.1002/mnfr.202100650. Epub 2021 Oct 19.
To study the effect of variation in dietary vitamin A (VA) content on its hepatic and intestinal metabolism.
Adult female and male rats are fed with diets containing 400, 2300, or 9858 IU kg VA for 31-33 weeks. VA concentrations are measured in plasma and liver. Bioavailability and intestinal conversion efficiency of β-carotene to VA are assessed by measuring postprandial plasma β-carotene and retinyl palmitate concentrations after force-feeding rats with β-carotene. Expression of genes involved in VA metabolism, together with concentrations of RBP4, BCO1, and SR-BI proteins, are measured in the intestine and liver of female rats. Plasma retinol concentrations are lower and hepatic free retinol concentrations are higher in females than in males. There is no effect of dietary VA content on β-carotene bioavailability and its conversion efficiency, but bioavailability is higher and conversion efficiency is lower in females than in males. The expression of most genes exhibited a U-shaped dose response curve depending on VA intake.
β-Carotene bioavailability and conversion efficiency to VA are affected by the sex of rats. Results of gene expression suggest a hormetic regulation of VA metabolism in female rats.
研究膳食维生素A（VA）含量变化对其肝脏和肠道代谢的影响。
成年雌性和雄性大鼠分别喂食含400、2300或9858国际单位/千克VA的饲料31 - 33周。测定血浆和肝脏中的VA浓度。通过给大鼠强制喂食β - 胡萝卜素后测量餐后血浆β - 胡萝卜素和视黄醇棕榈酸酯浓度，评估β - 胡萝卜素向VA的生物利用度和肠道转化效率。在雌性大鼠的肠道和肝脏中测量参与VA代谢的基因表达，以及RBP4、BCO1和SR - BI蛋白的浓度。雌性大鼠的血浆视黄醇浓度低于雄性，而肝脏游离视黄醇浓度高于雄性。膳食VA含量的VA含量对β - 胡萝卜素的生物利用度及其转化效率没有影响，但雌性大鼠的生物利用度高于雄性，而转化效率低于雄性。大多数基因的表达根据VA摄入量呈现出U形剂量反应曲线。
β - 胡萝卜素向VA的生物利用度和转化效率受大鼠性别的影响。基因表达结果表明雌性大鼠中VA代谢存在 hormetic 调节。 （注：“hormetic”可能是专业术语，暂未找到准确对应中文，保留英文）
