酵母苯丙氨酰 - tRNA合成酶氨酰化反应中的氨酰腺苷酸机制。
The aminoacyladenylate mechanism in the aminoacylation reaction of yeast phenylalanyl-tRNA synthetase.
作者信息
Fasiolo F, Fersht A R
出版信息
Eur J Biochem. 1978 Apr;85(1):85-8. doi: 10.1111/j.1432-1033.1978.tb12214.x.
Abstract
It is shown from a combination of rapid quenching and steady-state kinetics that the phenylalanyl-tRNA synthetase from yeast catalyses the formation of phenylalanyl-tRNA by the amino-acyladenylate pathway at pH 7.8 and 25 degrees C. The rate-determining step at saturating reagent concentrations is not the dissociation of the charged tRNA from the enzyme.
摘要
快速淬灭和稳态动力学相结合的研究表明，来自酵母的苯丙氨酰 - tRNA合成酶在pH 7.8和25摄氏度下通过氨酰腺苷酸途径催化苯丙氨酰 - tRNA的形成。在饱和试剂浓度下，速率决定步骤不是带电tRNA从酶上的解离。
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