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酵母苯丙氨酰 - tRNA合成酶催化tRNAPhe氨酰化的稳态机制分析。

Analysis of the steady-state mechanism of the aminoacylation of tRNAPhe by phenylalanyl-tRNA synthetase from yeast.

作者信息

Thiebe R

出版信息

Nucleic Acids Res. 1978 Jun;5(6):2055-71. doi: 10.1093/nar/5.6.2055.

DOI:10.1093/nar/5.6.2055
PMID:353737
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342144/
Abstract

The steady-state mechanism of the aminoacylation of tRNAPhe by the corresponding synthetase from yeast has been investigated in detail by kinetic experiments. It was found that there are two alternative mechanisms: one favoured at low tRNA concentrations and the other at high tRNA concentrations. ATP and Phe are bound randomly to the enzyme. AMP is released immediately after the binding of ATP and Phe. Between the release of AMP and pyrophosphate (PPi) there is at least one additional step. Based on the experimental results a model of the steady-state mechanism is proposed. This model includes the sequence of addition of substrates to the enzyme and the release of products from the enzyme as well as the composition of the intermediate complexes with the enzyme. This model is in accordance with previous results based on different techniques. The results are explained by a "flip-flop" mechanism for all the substrates and products involved in the reaction.

摘要

通过动力学实验，对酵母中相应合成酶催化苯丙氨酰 - tRNAPhe 的稳态机制进行了详细研究。结果发现存在两种替代机制：一种在低tRNA浓度下占优势，另一种在高tRNA浓度下占优势。ATP和苯丙氨酸随机结合到酶上。ATP和苯丙氨酸结合后，AMP立即释放。在AMP释放和焦磷酸（PPi）释放之间至少还有一个额外步骤。基于实验结果，提出了一个稳态机制模型。该模型包括底物添加到酶上的顺序、产物从酶上的释放以及与酶形成的中间复合物的组成。该模型与基于不同技术的先前结果一致。反应中涉及的所有底物和产物的结果都用“触发器”机制来解释。

相似文献

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Analysis of the steady-state mechanism of the aminoacylation of tRNAPhe by phenylalanyl-tRNA synthetase from yeast.酵母苯丙氨酰 - tRNA合成酶催化tRNAPhe氨酰化的稳态机制分析。
Nucleic Acids Res. 1978 Jun;5(6):2055-71. doi: 10.1093/nar/5.6.2055.
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Specific interaction of anticodon loop residues with yeast phenylalanyl-tRNA synthetase.反密码子环残基与酵母苯丙氨酰 - tRNA合成酶的特异性相互作用。
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A comparative study of the interactions of Escherichia coli leucyl-, seryl-, and valyl-transfer ribonucleic acid synthetases with their cognate transfer ribonucleic acids.大肠杆菌亮氨酰、丝氨酰和缬氨酰转移核糖核酸合成酶与其同源转移核糖核酸相互作用的比较研究。
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