Center for Biomedical Analysis, Department of Chemistry, College of Science, University of Sulaimani, Qliasan St, 46002, Slemani City, Kurdistan Region, Iraq.
Center for Biomedical Analysis, Department of Chemistry, College of Science, University of Sulaimani, Qliasan St, 46002, Slemani City, Kurdistan Region, Iraq.
Talanta. 2022 Jan 1;236:122878. doi: 10.1016/j.talanta.2021.122878. Epub 2021 Sep 11.
The development of diagnostic devices based on memetic molecular recognitions are becoming highly promising due to high specificity, sensitivity, stability, and low-cost comparing to natural molecular recognition. During the last decade, molecular imprinted polymers (MIPs) and aptamer have shown dramatic enhancement in the molecular recognition characteristics for bio(chemical) sensing applications. Recently, MIP-aptamer, as an emerging hybrid recognition element, merged the advantages of the both recognition components. This dual recognition-based sensor has shown improved properties and desirable features, such as high sensitivity, low limit of detection, high stability under harsh environmental conditions, high binding affinity, and superior selectivity. Hybrid MIP-aptamer as dual recognition element, was used in the real sample analysis, such as detection of proteins, neurotransmitters, environmental pollutants, biogenic compounds, small ions, explosives, virus detections and pharmaceuticals. This review focuses on a comprehensive overview of the preparation strategies of various MIP-aptamer recognition elements, mechanism of formation of MIP-aptamer, and detection of various target molecules in different matrices.
基于遗传分子识别的诊断设备的发展由于具有比天然分子识别更高的特异性、灵敏度、稳定性和低成本，因此具有很高的应用前景。在过去的十年中，分子印迹聚合物（MIP）和适体在生物（化学）传感应用中的分子识别特性方面取得了显著的提高。最近，MIP-适体作为一种新兴的混合识别元件，融合了两种识别组件的优势。这种基于双重识别的传感器具有改善的性能和理想的特点，例如高灵敏度、低检测限、在恶劣环境条件下的高稳定性、高结合亲和力和优异的选择性。混合 MIP-适体作为双重识别元件，已用于实际样品分析，例如蛋白质、神经递质、环境污染物、生物化合物、小离子、爆炸物、病毒检测和药物的检测。本文综述了各种 MIP-适体识别元件的制备策略、MIP-适体形成的机制以及不同基质中各种目标分子的检测。
