State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuan mingyuan West Road, Haidian District, Beijing, 100193, People's Republic of China.
The State Key Laboratory of Protein and Plant Gene Research, National Teaching Center for Experimental Biology, School of Life Sciences, Peking University, 100871, Beijing, China.
Arch Toxicol. 2021 Dec;95(12):3777-3786. doi: 10.1007/s00204-021-03158-1. Epub 2021 Oct 11.
Thifluzamide is widely used fungicide and frequently detected in aquatic system. In this study, the toxicity of fungicide thifluzamide to non-targeted aquatic organisms was investigated for neuroendocrine disruption potentials. Here, zebrafish embryos were exposed to a series of concentrations of thifluzamide for 6 days. The results showed that both the development of embryos/larvae and the behavior of hatched larvae were significantly affected by thifluzamide. Importantly, the decreased activity of acetylcholinesterase (AchE) and the increased contents of neurotransmitters such as serotonin (5-HT) and norepinephrine (NE), along with transcriptional changes of nervous system related genes were observed following 4 days exposure to thifluzamide. Besides, the decreased contents of triiodothyronine (T3) and thyroxine (T4) in whole body, as well as significant expression alteration in hypothalamic-pituitary-thyroid (HPT) axis associated genes were discovered in zebrafish embryos after 4 days of exposure to thifluzamide. Our results clearly demonstrated that zebrafish embryos exposed to thifluzamide could disrupt neuroendocrine, compromise behavior and induce developmental abnormality, suggesting impact of this fungicide on developmental programming in zebrafish.
噻呋酰胺是一种广泛使用的杀菌剂，经常在水生系统中检测到。在这项研究中，研究了杀菌剂噻呋酰胺对非靶标水生生物的神经内分泌干扰潜力的毒性。在这里，将斑马鱼胚胎暴露于一系列噻呋酰胺浓度下 6 天。结果表明，噻呋酰胺显著影响胚胎/幼虫的发育和孵化后的幼虫的行为。重要的是，在暴露于噻呋酰胺 4 天后，观察到乙酰胆碱酯酶（AchE）活性降低，以及神经递质 5-羟色胺（5-HT）和去甲肾上腺素（NE）等的含量增加，以及神经系统相关基因的转录变化。此外，在暴露于噻呋酰胺 4 天后，在斑马鱼胚胎的整个身体中发现三碘甲状腺原氨酸（T3）和甲状腺素（T4）的含量降低，以及下丘脑-垂体-甲状腺（HPT）轴相关基因的表达明显改变。我们的结果清楚地表明，暴露于噻呋酰胺的斑马鱼胚胎会破坏神经内分泌，损害行为并诱导发育异常，这表明这种杀菌剂对斑马鱼的发育编程有影响。
