Electroanalytical Lab, Department of Chemistry, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur 522502, Andhra Pradesh, India.
Electroanalytical Lab, Department of Chemistry, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur 522502, Andhra Pradesh, India.
Bioelectrochemistry. 2022 Feb;143:107935. doi: 10.1016/j.bioelechem.2021.107935. Epub 2021 Aug 21.
Serum 25-hydroxyvitamin D (25(OH)D) has been clinically considered as a novel biomarker for vitamin D deficiency. The current standard technologies for the detection of 25(OH)D are performed in sophisticated laboratories exhibiting the practical limitations for onsite and affordable testing. Therefore, the development of a cost-effective device for Vitamin D is extremely necessary to provide an earlier diagnosis. Herein, for the first time, we propose a novel label-free impedimetric immunosensor for the detection and quantification of 25-hydroxyvitamin D (25(OH)D) biomarker in serum samples based on the Au nanoparticles functionalized GCN-β-CD nanocomposite. To fabricate the sensing probe, Ab-25(OH)D antibodies were covalently immobilized on GCN-β-CD@Au/GCE using carbodiimide chemistry. The surface morphology and structural properties of constructed immunosensor were confirmed by different analytical techniques. Electrochemical impedance spectroscopy technique (EIS) has been selected as the main detection method to measure the Antibody (Ab) and Antigen (Ag) interaction at the immunosensor surface because it is label-free, less destructive to the activities of the biomolecule, and highly sensitive. The as-prepared immunosensor exhibited an excellent concentration range from 0.1 ng/ml to 500 ng/ml with the lowest limit of detection of 0.01 ng/ml. Furthermore, the sensing probe was validated in serum samples and obtained results were compared with the standard CLIA technique. The results have revealed that the sensing probe could be used for clinical diagnosis of Vitamin D deficiency in the clinical laboratories.
血清 25-羟维生素 D（25(OH)D）已被临床视为维生素 D 缺乏症的新型生物标志物。目前检测 25(OH)D 的标准技术是在复杂的实验室中进行的，这些实验室存在现场和经济实惠测试的实际限制。因此，开发一种经济高效的维生素 D 检测设备对于提供早期诊断极为必要。在这里，我们首次提出了一种基于 Au 纳米粒子功能化 GCN-β-CD 纳米复合材料的新型无标记阻抗免疫传感器，用于检测和定量血清样品中的 25-羟维生素 D（25(OH)D）生物标志物。为了制备传感探针，使用碳二亚胺化学将 Ab-25(OH)D 抗体共价固定在 GCN-β-CD@Au/GCE 上。通过不同的分析技术证实了构建的免疫传感器的表面形貌和结构特性。电化学阻抗谱技术（EIS）已被选为主要检测方法，用于测量免疫传感器表面上的抗体（Ab）和抗原（Ag）相互作用，因为它是无标记的，对生物分子的活性破坏较小，并且具有高度的敏感性。所制备的免疫传感器表现出从 0.1ng/ml 到 500ng/ml 的优异浓度范围，最低检测限为 0.01ng/ml。此外，该传感探针在血清样本中进行了验证，并将结果与标准 CLIA 技术进行了比较。结果表明，该传感探针可用于临床实验室维生素 D 缺乏症的临床诊断。
