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在 中全基因组鉴定和 BZR 转录因子基因家族的表达谱分析。

Genome-Wide Identification and Expression Profiling of the BZR Transcription Factor Gene Family in .

机构信息

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.

College of Life Science, China Jiliang University, Hangzhou 310058, China.

出版信息

Int J Mol Sci. 2021 Sep 26;22(19):10379. doi: 10.3390/ijms221910379.

Abstract

Brassinazole-resistant (BZR) family genes encode plant-specific transcription factors (TFs), play essential roles in the regulation of plant growth and development, and have multiple stress-resistance functions. is a model plant widely used in basic research. However, members of the BZR family in have not been identified, and little is known about their function in abiotic stress. In this study, a total of 14 BZR members were identified in the genome, which could be divided into four groups according to a phylogenetic tree. have similar exon-intron structures and conserved motifs, and may be regulated by cis-acting elements such as STRE, TCA, and ARE, etc. Organ-specific expression analysis showed that members have different and diverse expression patterns in different tissues, and most of the members are expressed in roots, stems, and leaves. The analysis of the expression patterns in response to different abiotic stresses showed that all the tested members showed a significant down-regulation after drought treatment. Many genes also responded in various ways to cold, heat and salt stress treatments. The results imply that NbBZRs have multiple functions related to stress resistance.

摘要

油菜素内酯不敏感(BZR)家族基因编码植物特异性转录因子(TFs),在植物生长发育的调控中发挥着重要作用,具有多种抗逆性功能。油菜是一种广泛应用于基础研究的模式植物。然而,油菜素内酯不敏感家族的成员在油菜中尚未被鉴定,其在非生物胁迫中的功能也知之甚少。本研究在油菜基因组中鉴定出了 14 个 BZR 成员,根据系统发育树可将其分为四个组。油菜素内酯不敏感成员具有相似的外显子-内含子结构和保守基序,可能受到 STRE、TCA 和 ARE 等顺式作用元件的调控。组织特异性表达分析表明,油菜素内酯不敏感成员在不同组织中具有不同和多样化的表达模式,大多数成员在根、茎和叶中表达。对不同非生物胁迫响应的表达模式分析表明,所有测试的油菜素内酯不敏感成员在干旱处理后均表现出显著下调。许多油菜素内酯不敏感基因也对冷、热和盐胁迫处理有不同的响应方式。这些结果表明,NbBZRs 具有与抗逆性相关的多种功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbb0/8508657/5c711929b285/ijms-22-10379-g001.jpg

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