拟南芥 Fasciclin-like 阿拉伯半乳糖蛋白基因家族：在 Nicotiana benthamiana 中的全基因组鉴定、分类和对病原体的表达反应。

Fasciclin-like arabinogalactan gene family in Nicotiana benthamiana: genome-wide identification, classification and expression in response to pathogens.

机构信息

College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.

出版信息

BMC Plant Biol. 2020 Jul 1;20(1):305. doi: 10.1186/s12870-020-02501-5.

BACKGROUND

Nicotiana benthamiana is widely used as a model plant to study plant-pathogen interactions. Fasciclin-like arabinogalactan proteins (FLAs), a subclass of arabinogalactan proteins (AGPs), participate in mediating plant growth, development and response to abiotic stress. However, the members of FLAs in N. benthamiana and their response to plant pathogens are unknown.

RESULTS

38 NbFLAs were identified from a genome-wide study. NbFLAs could be divided into four subclasses, and their gene structure and motif composition were conserved in each subclass. NbFLAs may be regulated by cis-acting elements such as STRE and MBS, and may be the targets of transcription factors like C2H2. Quantitative real time polymerase chain reaction (RT-qPCR) results showed that selected NbFLAs were differentially expressed in different tissues. All of the selected NbFLAs were significantly downregulated following infection by turnip mosaic virus (TuMV) and most of them also by Pseudomonas syringae pv tomato strain DC3000 (Pst DC3000), suggesting possible roles in response to pathogenic infection.

CONCLUSIONS

This study systematically identified FLAs in N. benthamiana, and indicates their potential roles in response to biotic stress. The identification of NbFLAs will facilitate further studies of their role in plant immunity in N. benthamiana.

背景

黄花烟（Nicotiana benthamiana）被广泛用作研究植物-病原体相互作用的模式植物。纤维连接蛋白样阿拉伯半乳聚糖蛋白（FLAs）是阿拉伯半乳聚糖蛋白（AGPs）的一个亚类，参与介导植物的生长、发育和对非生物胁迫的反应。然而，黄花烟中的 FLA 成员及其对植物病原体的反应尚不清楚。

结果

通过全基因组研究鉴定了 38 个 NbFLAs。NbFLAs 可分为四个亚类，每个亚类的基因结构和基序组成都保守。NbFLAs 可能受到顺式作用元件（如 STRE 和 MBS）的调控，也可能是 C2H2 等转录因子的靶标。定量实时聚合酶链反应（RT-qPCR）结果表明，选定的 NbFLAs 在不同组织中表达水平存在差异。感染芜菁花叶病毒（TuMV）和大多数 Pst DC3000 后，所有选定的 NbFLAs 均显著下调，表明它们可能在应对致病感染中发挥作用。