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泛素/生长素诱导蛋白基因家族和生长素响应因子基因家族的全基因组鉴定和表达分析。

Genome-Wide Identification and Expression Analysis of the Aux/IAA and Auxin Response Factor Gene Family in .

机构信息

Guangdong Engineering Research Center for Grassland Science, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.

College of Grassland Science, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Int J Mol Sci. 2021 Sep 28;22(19):10494. doi: 10.3390/ijms221910494.

Abstract

and auxin response transcription factor () genes are key regulators of auxin responses in plants. A total of 25 and 40 genes were identified based on the latest updated reference genome sequence. They were clustered into 10 and 8 major groups, respectively. The homologs among , soybean, and shared close relationships based on phylogenetic analysis. Gene structure analysis revealed that and genes contained one to four concern motifs and they are localized to eight chromosomes, except chromosome 6 without In addition, some and genes were expressed in all tissues, while others were specifically expressed in specific tissues. Analysis of -acting elements in promoter region and expression profiles revealed the potential response of and genes to hormones and abiotic stresses. The prediction protein-protein interaction network showed that some ARF proteins could interact with multiple Aux/IAA proteins, and the reverse is also true. The investigation provides valuable, basic information for further studies on the biological functions of and genes in the regulation of auxin-related pathways in .

摘要

生长素响应转录因子(ARF)基因是植物中生长素响应的关键调节剂。基于最新更新的参考基因组序列,共鉴定出 25 个拟南芥和 40 个大豆 ARF 基因。它们分别聚类为 10 个和 8 个主要组。基于系统发育分析,拟南芥、大豆和棉花之间的同源物关系密切。基因结构分析表明,ARF 基因包含一到四个结构域,它们定位于 8 条染色体上,除了 6 号染色体没有。此外,一些 ARF 基因在所有组织中表达,而其他基因则在特定组织中特异性表达。启动子区顺式作用元件分析和表达谱分析表明,ARF 基因对激素和非生物胁迫有潜在的响应。预测的蛋白质-蛋白质互作网络表明,一些 ARF 蛋白可以与多个 Aux/IAA 蛋白相互作用,反之亦然。该研究为进一步研究 ARF 基因在调控生长素相关途径中的生物学功能提供了有价值的基础信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a7a/8532000/d7525472ce6e/ijms-22-10494-g001.jpg

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