Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry and Czech Advanced Technology and Research Institute, Palacký University Olomouc, Hněvotínská 5, 779 00 Olomouc, Czech Republic.
Int J Mol Sci. 2021 Oct 5;22(19):10759. doi: 10.3390/ijms221910759.
Synchronous cell populations are commonly used for the analysis of various aspects of cellular metabolism at specific stages of the cell cycle. Cell synchronization at a chosen cell cycle stage is most frequently achieved by inhibition of specific metabolic pathway(s). In this respect, various protocols have been developed to synchronize cells in particular cell cycle stages. In this review, we provide an overview of the protocols for cell synchronization of mammalian cells based on the inhibition of synthesis of DNA building blocks-deoxynucleotides and/or inhibition of DNA synthesis. The mechanism of action, examples of their use, and advantages and disadvantages are described with the aim of providing a guide for the selection of suitable protocol for different studied situations.
同步细胞群体通常用于分析细胞周期特定阶段细胞代谢的各个方面。通过抑制特定的代谢途径来实现细胞在选定的细胞周期阶段的同步化。在这方面，已经开发了各种方案来将细胞同步到特定的细胞周期阶段。在这篇综述中，我们提供了基于抑制 DNA 构建块（脱氧核苷酸）的合成和/或 DNA 合成抑制的哺乳动物细胞同步化方案的概述。描述了作用机制、使用实例以及优缺点，旨在为不同研究情况选择合适方案提供指导。
