鸡组蛋白H5球状结构域的核磁共振研究：共振归属与二级结构
Nuclear magnetic resonance study of the globular domain of chicken histone H5: resonance assignment and secondary structure.
作者信息
Zarbock J, Clore G M, Gronenborn A M
出版信息
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7628-32. doi: 10.1073/pnas.83.20.7628.
Abstract
A 1H NMR study of the 79-residue globular domain of chicken erythrocyte histone H5 (GH5) is presented. Using a combination of two-dimensional NMR techniques to demonstrate through-bond and through-space (less than 5 A) connectivities, the resonances of GH5 are assigned in a sequential manner. From a qualitative interpretation of the short-range nuclear Overhauser effects (NOEs) involving the NH and C alpha H protons, it is shown that GH5 has four alpha-helices. The approximate spatial relationship of three of these four helices relative to each other is deduced from the observation of a number of long-range NOEs. The peptide chain outside the helices appears to have little regular secondary structure and no NOEs characteristic of beta-sheets are apparent.
摘要
本文介绍了对鸡红细胞组蛋白H5（GH5）79个残基球状结构域的1H NMR研究。通过结合二维NMR技术来证明键间和空间（小于5埃）连接性，以序列方式对GH5的共振进行了归属。通过对涉及NH和CαH质子的短程核Overhauser效应（NOE）的定性解释，表明GH5有四个α螺旋。从一些长程NOE的观察中推断出这四个螺旋中三个彼此之间的近似空间关系。螺旋外的肽链似乎几乎没有规则的二级结构，也没有明显的β折叠特征的NOE。
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本文引用的文献
1
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2
Chicken erythrocyte histone H5. IV. Sequence of the carboxy-termined half of the molecule (96 residues) and complete sequence.鸡红细胞组蛋白H5。IV。该分子羧基末端一半（96个残基）的序列及完整序列。
FEBS Lett. 1980 Apr 7;112(2):147-51. doi: 10.1016/0014-5793(80)80167-0.
3
Sequential resonance assignments as a basis for determination of spatial protein structures by high resolution proton nuclear magnetic resonance.作为通过高分辨率质子核磁共振确定蛋白质空间结构基础的序列共振归属。
J Mol Biol. 1982 Mar 5;155(3):311-9. doi: 10.1016/0022-2836(82)90007-9.
4
Nucleosome structure.核小体结构。
Annu Rev Biochem. 1980;49:1115-56. doi: 10.1146/annurev.bi.49.070180.005343.
5
Improved spectral resolution in cosy 1H NMR spectra of proteins via double quantum filtering.通过双量子滤波提高蛋白质的 cosy 1H NMR 谱中的光谱分辨率。
Biochem Biophys Res Commun. 1983 Dec 16;117(2):479-85. doi: 10.1016/0006-291x(83)91225-1.
6
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