Nguyen Son Hai, Nguyen Van-Nhat, Tran Mai Thi
School of Mechanical Engineering, Hanoi University of Science and Technology, Hanoi, 100000, Viet Nam.
College of Engineering and Computer Science, VinUniversity, Hanoi, 100000, Viet Nam.
Heliyon. 2024 May 21;10(10):e31617. doi: 10.1016/j.heliyon.2024.e31617. eCollection 2024 May 30.
The detection of ampicillin plays a crucial role in managing and monitoring its usage and resistance. This study introduces a simple and effective biosensor for ampicillin detection, utilizing the unique absorbance features of Mn-doped ZnS capped by chitosan micromaterials in conjunction with β-lactamase activity. The biosensors can detect ampicillin concentrations from 13.1 to 72.2 μM, with a minimum detection limit of 2.93 μM for sensors based on 300 mg/L of the sensing material. In addition, these sensors show high specificity for ampicillin over other antibiotics such as penicillin, tetracycline, amoxicillin, cephalexin, and a non-antibiotic-glucose. This specificity is demonstrated by an enhancing effect when beta-lactamase is used, as opposed to a quenching effect observed at 340 nm in the absorbance spectrum when no beta-lactamase is present. This research highlights the potential of affordable chitosan-capped Mn-doped ZnS micromaterials for detecting ampicillin through simple absorbance measurements, which could improve the monitoring of antibiotics in both clinical and environmental settings.
氨苄西林的检测在其使用管理和耐药性监测中起着至关重要的作用。本研究介绍了一种用于检测氨苄西林的简单有效的生物传感器，该传感器利用壳聚糖微材料包覆的锰掺杂硫化锌的独特吸光度特性，并结合β-内酰胺酶活性。该生物传感器能够检测13.1至72.2 μM的氨苄西林浓度，对于基于300 mg/L传感材料的传感器，其最低检测限为2.93 μM。此外，这些传感器对氨苄西林的特异性高于其他抗生素，如青霉素、四环素、阿莫西林、头孢氨苄以及非抗生素葡萄糖。当使用β-内酰胺酶时会产生增强效应，这证明了其特异性，而在没有β-内酰胺酶时，在吸光度光谱的340 nm处观察到淬灭效应。这项研究突出了价格低廉的壳聚糖包覆锰掺杂硫化锌微材料通过简单吸光度测量检测氨苄西林的潜力，这可能会改善临床和环境中抗生素的监测。
