Hatamie Shadie, Shih Po-Jen, Chen Bo-Wei, Shih Hua-Ju, Wang I-Jong, Young Tai-Horng, Yao Da-Jeng
College of Medicine, National Taiwan University, Taipei 10048, Taiwan.
Department of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Polymers (Basel). 2021 Sep 28;13(19):3306. doi: 10.3390/polym13193306.
To improve bovine corneal endothelial cell (BCEC) migration, enhance cell energy, and facilitate symmetric cell distribution in corneal surfaces, an electromagnet device was fabricated. Twenty nanometer superparamagnetic iron oxide nanoparticles (SPIONs) functionalized with fourth-generation dendrimer macromolecules were synthesized, and their size and structure were evaluated using transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR). The results confirmed the configuration of the dendrimer on the SPION surfaces. In vitro biocompatibility was assessed using the 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl tetrazolium bromide assay. No significant toxicity was noted on BCECs within 24 h of incubation. In the cell migration assay, cells treated with dendrimer-coated SPIONs exhibited a relatively high wound healing rate under sample addition (1 μg/mL) under a magnetic field. Real-time PCR on BCECs treated with dendrimer-coated SPIONs revealed upregulation of specific genes, including AT1P1 and NCAM1, for BCECs-dendrimer-coated SPIONs under a magnetic field. The three-dimensional dispersion of BCECs containing dendrimer-coated SPIONs under a magnetic field was evaluated using COMSOL Multiphysics software. The results revealed the BCECs-SPION vortex pattern layers in the corneal surface corresponded to the electromagnet's displacement from the ocular surface. Magnetic resonance imaging (MRI) indicated that dendrimer-coated SPIONs can be used as a T contrast agent.
为了促进牛角膜内皮细胞（BCEC）迁移、增强细胞能量并促进角膜表面细胞的对称分布，制造了一种电磁装置。合成了用第四代树枝状大分子功能化的20纳米超顺磁性氧化铁纳米颗粒（SPIONs），并使用透射电子显微镜（TEM）、X射线光电子能谱（XPS）和傅里叶变换红外光谱（FTIR）对其大小和结构进行了评估。结果证实了树枝状大分子在SPION表面的构型。使用3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐试验评估了体外生物相容性。在孵育24小时内，未发现对BCECs有明显毒性。在细胞迁移试验中，在磁场下添加样品（1μg/mL）时，用树枝状大分子包被的SPIONs处理的细胞表现出相对较高的伤口愈合率。对用树枝状大分子包被的SPIONs处理的BCECs进行实时PCR，结果显示在磁场下，BCECs-树枝状大分子包被的SPIONs的特定基因（包括AT1P1和NCAM1）上调。使用COMSOL Multiphysics软件评估了在磁场下含有树枝状大分子包被的SPIONs的BCECs的三维分散情况。结果显示，角膜表面的BCECs-SPION涡旋图案层与电磁体相对于眼表面的位移相对应。磁共振成像（MRI）表明，树枝状大分子包被的SPIONs可用作T造影剂。
