Wickramasinghe S N
Alcohol Alcohol. 1986;21(3):263-8.
After macrophages are cultured in the presence of ethanol (0.1-2.0 mg/ml) for 72 hr, the culture supernatant contains one or more cytotoxic factors which cause the detachment of mouse A9 tumour cells and a reduction of 3H-thymidine incorporation by K562 cells. The cytotoxic activity is largely abolished by heating to 56 degrees C for 10 min and is non-dialysable and hence associated with material having a molecular weight greater than about 10,000. These in vitro data support the possibility that alcohol-induced tissue damage may result from the action of cytotoxic factors generated as a consequence of an interaction between ethanol and tissue macrophages in vivo.
巨噬细胞在含有乙醇（0.1 - 2.0毫克/毫升）的条件下培养72小时后，培养上清液中含有一种或多种细胞毒性因子，这些因子会导致小鼠A9肿瘤细胞脱离，并使K562细胞的3H - 胸腺嘧啶核苷掺入量减少。将其加热至56摄氏度10分钟后，细胞毒性活性基本消失，且该活性不可透析，因此与分子量大于约10,000的物质有关。这些体外实验数据支持了这样一种可能性，即酒精诱导的组织损伤可能是由于体内乙醇与组织巨噬细胞相互作用产生的细胞毒性因子的作用所致。
