Dep. of Plant Sciences, Univ. of Saskatchewan, Saskatoon, SK, Canada.
School of Natural Sciences, Univ. of Tasmania, Hobart, TAS, Australia.
Plant Genome. 2021 Nov;14(3):e20144. doi: 10.1002/tpg2.20144. Epub 2021 Oct 13.
The continued success of lentil (Lens culinaris Medik.) genetic improvement relies on the availability of broad genetic diversity, and new alleles need to be identified and incorporated into the cultivated gene pool. Availability of robust and predictive markers greatly enhances the precise transfer of genomic regions from unadapted germplasm. Quantitative trait loci (QTL) for key phenological traits in lentil were located using a recombinant inbreed line (RIL) population derived from a cross between an Ethiopian landrace (ILL 1704) and a northern temperate cultivar (CDC Robin). Field experiments were conducted at Sutherland research farm in Saskatoon and at Rosthern, Saskatchewan, Canada during 2018 and 2019. A linkage map was constructed using 21,634 single nucleotide polymorphisms (SNPs) located on seven linkage groups (LGs), which correspond to the seven haploid chromosomes of lentil. Eight QTL were identified for six phenological traits. Flowering-related QTL were identified at two regions on LG6. FLOWERING LOCUS T (FT) genes were annotated within the flowering time QTL interval based on the lentil reference genome. Similarly, a major QTL for postflowering developmental processes was located on LG5 with several senescence-associated genes annotated within the QTL interval. The flowering time QTL was validated in a different genetic background indicating the potential use of the identified markers for marker-assisted selection to precisely transfer genomic regions from exotic germplasm into elite crop cultivars without disrupting adaptation.
菜豆（Lens culinaris Medik.）遗传改良的持续成功依赖于广泛遗传多样性的可用性，需要鉴定新的等位基因并将其纳入栽培基因库。稳健且可预测的标记的可用性极大地增强了从非适应种质中精确转移基因组区域的能力。使用埃塞俄比亚地方品种（ILL 1704）和北温带栽培品种（CDC Robin）杂交衍生的重组自交系（RIL）群体，定位了菜豆关键表型性状的数量性状位点（QTL）。田间试验分别于 2018 年和 2019 年在萨斯卡通的萨瑟兰研究农场和萨斯喀彻温省的罗斯特恩进行。使用位于七个连锁群（LG）上的 21,634 个单核苷酸多态性（SNP）构建了连锁图谱，这与菜豆的七个单倍体染色体相对应。鉴定了 6 个表型性状的 8 个 QTL。在 LG6 上的两个区域鉴定到与开花相关的 QTL。基于菜豆参考基因组，在开花时间 QTL 区间内注释了开花时间相关基因 FLOWERING LOCUS T（FT）。同样，在 LG5 上定位到一个主要的开花后发育过程 QTL，在 QTL 区间内注释了几个与衰老相关的基因。在不同遗传背景下验证了开花时间 QTL，表明所鉴定的标记可用于标记辅助选择，将来自外来种质的基因组区域精确转移到优良作物品种中，而不会破坏适应性。
