大肠杆菌中上下文对无义密码子抑制的影响。
Context effects on nonsense codon suppression in Escherichia coli.
作者信息
Feinstein S I, Altman S
出版信息
Genetics. 1978 Feb;88(2):201-19. doi: 10.1093/genetics/88.2.201.
Abstract
The influence of mRNA context on nonsense codon suppression has been studied by suppression measurements at one site in the Escherichia coli trpE gene and at two sites in the trpA gene. The ratio of suppression efficiencies of amber and ochre codons at each site (homotopic pairs) has been compared using ochre suppressing derivatives of tRNATyr. This ratio is independent of differential effects of the inserted amino acid on enzyme function. We have found that mRNA context can change the ratio of suppression efficiencies of homotopic nonsense codons at the three sites in the trp gene system over a ten-fold range. The causes of such variation, and, in particular the effect of certain adjacent nucleotides on nonsense codon suppression are considered.
摘要
通过在大肠杆菌色氨酸合成酶E基因的一个位点以及色氨酸合成酶A基因的两个位点进行抑制测量，研究了mRNA上下文对无义密码子抑制的影响。使用tRNATyr的赭石抑制衍生物，比较了每个位点（同位对）琥珀密码子和赭石密码子的抑制效率之比。该比例与插入氨基酸对酶功能的差异效应无关。我们发现，mRNA上下文可使色氨酸基因系统中三个位点的同位无义密码子抑制效率之比在10倍范围内发生变化。文中考虑了这种变化的原因，特别是某些相邻核苷酸对无义密码子抑制的影响。
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