Martin R, Weiner M, Gallant J
Department of Genetics, University of Washington, Seattle 98195.
J Bacteriol. 1988 Oct;170(10):4714-7. doi: 10.1128/jb.170.10.4714-4717.1988.
In Escherichia coli, nonsense suppression at UAA codons is governed by the competition between a suppressor tRNA and the translational release factors RF1 and RF2. We have employed plasmids carrying the genes for RF1 and RF2 to measure release factor preference at UAA codons at 13 different sites in the lacI gene. We show here that the activity of RF1 and RF2 varies according to messenger context. RF1 is favored at UAA codons which are efficiently suppressed. RF2 is preferred at poorly suppressed sites.
在大肠杆菌中，UAA密码子处的无义抑制受抑制性tRNA与翻译释放因子RF1和RF2之间竞争的调控。我们利用携带RF1和RF2基因的质粒来测定lacI基因13个不同位点处UAA密码子的释放因子偏好性。我们在此表明，RF1和RF2的活性根据信使上下文而变化。在被有效抑制的UAA密码子处，RF1占优势。在抑制效果差的位点，RF2更受青睐。
