释放因子背景对大肠杆菌中UAA密码子的影响。
Effects of release factor context at UAA codons in Escherichia coli.
作者信息
Martin R, Weiner M, Gallant J
机构信息
Department of Genetics, University of Washington, Seattle 98195.
出版信息
J Bacteriol. 1988 Oct;170(10):4714-7. doi: 10.1128/jb.170.10.4714-4717.1988.
Abstract
In Escherichia coli, nonsense suppression at UAA codons is governed by the competition between a suppressor tRNA and the translational release factors RF1 and RF2. We have employed plasmids carrying the genes for RF1 and RF2 to measure release factor preference at UAA codons at 13 different sites in the lacI gene. We show here that the activity of RF1 and RF2 varies according to messenger context. RF1 is favored at UAA codons which are efficiently suppressed. RF2 is preferred at poorly suppressed sites.
摘要
在大肠杆菌中，UAA密码子处的无义抑制受抑制性tRNA与翻译释放因子RF1和RF2之间竞争的调控。我们利用携带RF1和RF2基因的质粒来测定lacI基因13个不同位点处UAA密码子的释放因子偏好性。我们在此表明，RF1和RF2的活性根据信使上下文而变化。在被有效抑制的UAA密码子处，RF1占优势。在抑制效果差的位点，RF2更受青睐。
相似文献
1
Effects of release factor context at UAA codons in Escherichia coli.释放因子背景对大肠杆菌中UAA密码子的影响。
J Bacteriol. 1988 Oct;170(10):4714-7. doi: 10.1128/jb.170.10.4714-4717.1988.
2
Release factor competition is equivalent at strong and weakly suppressed nonsense codons.释放因子竞争在强抑制和弱抑制的无义密码子处是等效的。
Mol Gen Genet. 1988 Jul;213(1):144-9. doi: 10.1007/BF00333411.
3
A tripeptide 'anticodon' deciphers stop codons in messenger RNA.一种三肽“反密码子”可解读信使核糖核酸中的终止密码子。
Nature. 2000 Feb 10;403(6770):680-4. doi: 10.1038/35001115.
4
Functional interaction between release factor one and P-site peptidyl-tRNA on the ribosome.释放因子1与核糖体P位点肽基-tRNA之间的功能相互作用。
J Mol Biol. 1996 Aug 16;261(2):98-107. doi: 10.1006/jmbi.1996.0444.
5
Coevolution between Stop Codon Usage and Release Factors in Bacterial Species.细菌物种中终止密码子使用与释放因子之间的协同进化
Mol Biol Evol. 2016 Sep;33(9):2357-67. doi: 10.1093/molbev/msw107. Epub 2016 Jun 13.
6
Rapid and precise mapping of the Escherichia coli release factor genes by two physical approaches.通过两种物理方法对大肠杆菌释放因子基因进行快速精确的定位
J Bacteriol. 1988 Oct;170(10):4537-41. doi: 10.1128/jb.170.10.4537-4541.1988.
7
Single amino acid substitution in prokaryote polypeptide release factor 2 permits it to terminate translation at all three stop codons.原核生物多肽释放因子2中的单个氨基酸取代使其能够在所有三个终止密码子处终止翻译。
Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):8165-9. doi: 10.1073/pnas.95.14.8165.
8
Methylation of bacterial release factors RF1 and RF2 is required for normal translation termination in vivo.细菌释放因子RF1和RF2的甲基化是体内正常翻译终止所必需的。
J Biol Chem. 2007 Dec 7;282(49):35638-45. doi: 10.1074/jbc.M706076200. Epub 2007 Oct 10.
9
R213I mutation in release factor 2 (RF2) is one step forward for engineering an omnipotent release factor in bacteria .释放因子2（RF2）中的R213I突变是在细菌中构建全能释放因子方面向前迈出的一步。
J Biol Chem. 2017 Sep 8;292(36):15134-15142. doi: 10.1074/jbc.M117.785238. Epub 2017 Jul 25.
10
Stop codon recognition and interactions with peptide release factor RF3 of truncated and chimeric RF1 and RF2 from Escherichia coli.来自大肠杆菌的截短型和嵌合型RF1及RF2的终止密码子识别以及与肽释放因子RF3的相互作用。
Mol Microbiol. 2003 Dec;50(5):1467-76. doi: 10.1046/j.1365-2958.2003.03799.x.
引用本文的文献
1
Regulation of release factor expression using a translational negative feedback loop: a systems analysis.利用翻译负反馈环调控释放因子表达：系统分析。
RNA. 2012 Dec;18(12):2320-34. doi: 10.1261/rna.035113.112. Epub 2012 Oct 25.
2
Comparison of characteristics and function of translation termination signals between and within prokaryotic and eukaryotic organisms.原核生物和真核生物之间以及内部翻译终止信号的特征与功能比较。
Nucleic Acids Res. 2006 Apr 13;34(7):1959-73. doi: 10.1093/nar/gkl074. Print 2006.
3
Role of ribosome recycling factor (RRF) in translational coupling.核糖体循环因子（RRF）在翻译偶联中的作用。
EMBO J. 2000 Jul 17;19(14):3788-98. doi: 10.1093/emboj/19.14.3788.
4
Termination of translation in bacteria may be modulated via specific interaction between peptide chain release factor 2 and the last peptidyl-tRNA(Ser/Phe).细菌中的翻译终止可通过肽链释放因子2与最后一个肽基 - tRNA（丝氨酸/苯丙氨酸）之间的特异性相互作用来调节。
Nucleic Acids Res. 1993 Jun 25;21(12):2891-7. doi: 10.1093/nar/21.12.2891.
5
The second to last amino acid in the nascent peptide as a codon context determinant.新生肽中倒数第二个氨基酸作为密码子上下文决定因素。
EMBO J. 1994 Jan 1;13(1):249-57. doi: 10.1002/j.1460-2075.1994.tb06255.x.
6
The identity of the base following the stop codon determines the efficiency of in vivo translational termination in Escherichia coli.终止密码子之后的碱基的身份决定了大肠杆菌体内翻译终止的效率。
EMBO J. 1995 Jan 3;14(1):151-8. doi: 10.1002/j.1460-2075.1995.tb06985.x.
7
Context specific misreading of phenylalanine codons.苯丙氨酸密码子的上下文特异性错读。
Mol Gen Genet. 1989 Sep;218(3):397-401. doi: 10.1007/BF00332401.
8
Errors and alternatives in reading the universal genetic code.通用遗传密码解读中的错误与变体
Microbiol Rev. 1989 Sep;53(3):273-98. doi: 10.1128/mr.53.3.273-298.1989.
9
Aminoglycoside suppression at UAG, UAA and UGA codons in Escherichia coli and human tissue culture cells.氨基糖苷类药物对大肠杆菌和人组织培养细胞中UAG、UAA和UGA密码子的抑制作用。
Mol Gen Genet. 1989 Jun;217(2-3):411-8. doi: 10.1007/BF02464911.
10
Codon context.密码子上下文
Experientia. 1990 Dec 1;46(11-12):1126-33. doi: 10.1007/BF01936922.
本文引用的文献
1
The influence of codon context on genetic code translation.密码子上下文对遗传密码翻译的影响。
Nature. 1980 Jul 10;286(5769):123-7. doi: 10.1038/286123a0.
2
A temperature-sensitive mutant of Escherichia coli that shows enhanced misreading of UAG/A and increased efficiency for some tRNA nonsense suppressors.一种大肠杆菌的温度敏感突变体，其对UAG/A的错读增强，并且某些tRNA无义抑制子的效率提高。
Mol Gen Genet. 1984;193(1):38-45. doi: 10.1007/BF00327411.
3
Defined set of cloned termination suppressors: in vivo activity of isogenetic UAG, UAA, and UGA suppressor tRNAs.克隆终止抑制因子的特定集合：同基因UAG、UAA和UGA抑制性tRNA的体内活性
J Bacteriol. 1984 Jun;158(3):849-59. doi: 10.1128/jb.158.3.849-859.1984.
4
Genetic screen for cloned release factor genes.克隆释放因子基因的遗传筛选。
J Bacteriol. 1984 Apr;158(1):362-4. doi: 10.1128/jb.158.1.362-364.1984.
5
Context effects: translation of UAG codon by suppressor tRNA is affected by the sequence following UAG in the message.上下文效应：抑制性tRNA对UAG密码子的翻译受到信使RNA中UAG之后序列的影响。
J Mol Biol. 1983 Feb 15;164(1):73-87. doi: 10.1016/0022-2836(83)90088-8.
6
Effects of surrounding sequence on the suppression of nonsense codons.侧翼序列对无义密码子抑制的影响。
J Mol Biol. 1983 Feb 15;164(1):59-71. doi: 10.1016/0022-2836(83)90087-6.
7
Mapping and complementation studies of the gene for release factor 1.释放因子1基因的定位与互补研究。
J Bacteriol. 1986 Dec;168(3):1066-9. doi: 10.1128/jb.168.3.1066-1069.1986.
8
Nonsense suppression context effects in Escherichia coli bacteriophage T4.大肠杆菌噬菌体T4中的无义抑制上下文效应
Mol Gen Genet. 1987 May;207(2-3):517-8. doi: 10.1007/BF00331625.
9
Quantitative analysis of the relationship between nucleotide sequence and functional activity.核苷酸序列与功能活性之间关系的定量分析。
Nucleic Acids Res. 1986 Aug 26;14(16):6661-79. doi: 10.1093/nar/14.16.6661.
10
Influence of modification next to the anticodon in tRNA on codon context sensitivity of translational suppression and accuracy.tRNA中反密码子旁修饰对翻译抑制和准确性的密码子上下文敏感性的影响。
J Bacteriol. 1986 Jun;166(3):1022-7. doi: 10.1128/jb.166.3.1022-1027.1986.
Zotero 插件