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溴化乙锭的结合会导致核小体核心颗粒的解离。

Binding of ethidium bromide causes dissociation of the nucleosome core particle.

作者信息

McMurray C T, van Holde K E

出版信息

Proc Natl Acad Sci U S A. 1986 Nov;83(22):8472-6. doi: 10.1073/pnas.83.22.8472.

DOI:10.1073/pnas.83.22.8472
PMID:3464964
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC386952/
Abstract

The binding of ethidium bromide to chicken erythrocyte core particles results in a step-wise dissociation of the structure that involves the initial release of one copy each of histones H2A and H2B. Quantitation of the dissociated DNA reveals that a critical amount of drug is required for the dissociation. Above the critical value, the dissociation is time dependent, reversible, and independent of DNA concentration.

摘要

溴化乙锭与鸡红细胞核心颗粒的结合导致结构逐步解离，这涉及到组蛋白H2A和H2B各一个拷贝的初始释放。对解离的DNA进行定量分析表明，解离需要一定量的药物。超过临界值后，解离是时间依赖性的、可逆的，且与DNA浓度无关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/5e981f3d231a/pnas00326-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/aa601970c106/pnas00326-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/12287c33a1d7/pnas00326-0046-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/a9210706222e/pnas00326-0046-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/cf3b28318932/pnas00326-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/c6a7c95ba498/pnas00326-0047-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/7d4a79e2c2ce/pnas00326-0047-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/581d8a1a11f1/pnas00326-0047-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/5e981f3d231a/pnas00326-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/aa601970c106/pnas00326-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/12287c33a1d7/pnas00326-0046-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/a9210706222e/pnas00326-0046-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/cf3b28318932/pnas00326-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/c6a7c95ba498/pnas00326-0047-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/7d4a79e2c2ce/pnas00326-0047-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/581d8a1a11f1/pnas00326-0047-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24bd/386952/5e981f3d231a/pnas00326-0048-a.jpg

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The effect of ionic strength on DNA-ligand unwinding angles for acridine and quinoline derivatives.离子强度对吖啶和喹啉衍生物的DNA-配体解旋角的影响。
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A model for the association of intercalating ligands with mononucleosomes and chromatin.嵌入配体与单核小体和染色质结合的模型。
Trends Cancer. 2024 Aug;10(8):696-707. doi: 10.1016/j.trecan.2024.05.005. Epub 2024 Jun 1.
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Internal Motion of Chromatin Fibers Is Governed by Dynamics of Uncompressed Linker Strands.染色质纤维的内部运动受未压缩连接链动力学的支配。
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Rouse model with transient intramolecular contacts on a timescale of seconds recapitulates folding and fluctuation of yeast chromosomes.在秒级时间尺度上具有瞬时分子内接触的 Rouse 模型再现了酵母染色体的折叠和波动。
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A Comprehensive Biophysical Analysis of the Effect of DNA Binding Drugs on Protamine-induced DNA Condensation.DNA 结合药物对鱼精蛋白诱导的 DNA 凝聚的综合生物物理分析。
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