溴化乙锭的结合会导致核小体核心颗粒的解离。
Binding of ethidium bromide causes dissociation of the nucleosome core particle.
作者信息
McMurray C T, van Holde K E
出版信息
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8472-6. doi: 10.1073/pnas.83.22.8472.
Abstract
The binding of ethidium bromide to chicken erythrocyte core particles results in a step-wise dissociation of the structure that involves the initial release of one copy each of histones H2A and H2B. Quantitation of the dissociated DNA reveals that a critical amount of drug is required for the dissociation. Above the critical value, the dissociation is time dependent, reversible, and independent of DNA concentration.
摘要
溴化乙锭与鸡红细胞核心颗粒的结合导致结构逐步解离，这涉及到组蛋白H2A和H2B各一个拷贝的初始释放。对解离的DNA进行定量分析表明，解离需要一定量的药物。超过临界值后，解离是时间依赖性的、可逆的，且与DNA浓度无关。
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