Wang Xu, Jiang Bochen, Gu Lianfeng, Chen Yadi, Mora Manuel, Zhu Mulangma, Noory Eliace, Wang Qin, Lin Chentao
Basic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou, China.
Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA.
Nat Plants. 2021 Oct;7(10):1397-1408. doi: 10.1038/s41477-021-01002-z. Epub 2021 Oct 14.
Cryptochromes (CRYs) are photoreceptors that mediate light regulation of the circadian clock in plants and animals. Here we show that CRYs mediate blue-light regulation of N-methyladenosine (mA) modification of more than 10% of messenger RNAs in the Arabidopsis transcriptome, especially those regulated by the circadian clock. CRY2 interacts with three subunits of the METTL3/14-type N-methyladenosine RNA methyltransferase (mA writer): MTA, MTB and FIP37. Photo-excited CRY2 undergoes liquid-liquid phase separation (LLPS) to co-condense mA writer proteins in vivo, without obviously altering the affinity between CRY2 and the writer proteins. mta and cry1cry2 mutants share common defects of a lengthened circadian period, reduced mA RNA methylation and accelerated degradation of mRNA encoding the core component of the molecular oscillator circadian clock associated 1 (CCA1). These results argue for a photoregulatory mechanism by which light-induced phase separation of CRYs modulates mA writer activity, mRNA methylation and abundance, and the circadian rhythms in plants.
隐花色素（CRYs）是介导植物和动物生物钟光调节的光感受器。我们在此表明，CRYs介导拟南芥转录组中超过10%的信使RNA的N-甲基腺苷（mA）修饰的蓝光调节，尤其是那些受生物钟调节的信使RNA。CRY2与METTL3/14型N-甲基腺苷RNA甲基转移酶（mA写入器）的三个亚基相互作用：MTA、MTB和FIP37。光激发的CRY2在体内经历液-液相分离（LLPS）以共凝聚mA写入器蛋白，而不会明显改变CRY2与写入器蛋白之间的亲和力。mta和cry1cry2突变体具有昼夜节律延长、mA RNA甲基化降低以及编码分子振荡器生物钟相关1（CCA1）核心成分的mRNA加速降解的共同缺陷。这些结果支持了一种光调节机制，即光诱导的CRYs相分离调节mA写入器活性、mRNA甲基化和丰度以及植物的昼夜节律。
