Grădinaru Daniela, Margină Denisa, Ungurianu Anca, Nițulescu Georgiana, Pena Cătălina Monica, Ionescu-Tîrgoviște Constantin, Dănciulescu Miulescu Rucsandra
Department of Biochemistry, Faculty of Pharmacy, 'Carol Davila' University of Medicine and Pharmacy, 020956 Bucharest, Romania.
Department of Pharmaceutical Technology, Faculty of Pharmacy, 'Carol Davila' University of Medicine and Pharmacy, 020956 Bucharest, Romania.
Exp Ther Med. 2021 Dec;22(6):1393. doi: 10.3892/etm.2021.10829. Epub 2021 Sep 30.
Zinc deficiencies have been reported in numerous pathologies, such as diabetes mellitus, but also in the physiological process of ageing. Similarly, the end products of glycoxidation processes, advanced glycation end products (AGEs), are damaging compounds, a myriad of reports linking them to the development and progression of several age-associated chronic diseases. The aim of the present study was to analyze the relationships between zinc status, glycoxidative stress and insulin resistance (IR) in elderly subjects with type 2 diabetes mellitus (T2DM). A group of 52 non-smoking subjects (9 men and 43 women, aged 65-83 years) were enrolled in this cross-sectional study: 27 patients with T2DM, and 25 apparently healthy control subjects. Serum zinc (Zn) levels were assessed using a commercial kit based on an end-point colorimetric method, and serum AGEs were evaluated with a fluorimetric analytic procedure. The calculated glucose-to-zinc ratio (Gly/Zn), insulin-to-zinc ratio (Ins/Zn) and insulin-zinc resistance index (HOMA-IR/Zn) were further used to study the associations between serum Zn levels, secretory function of β-pancreatic cells and AGEs. T2DM patients presented significantly higher serum insulin and Zn levels, as compared to the controls. We found a significant inverse correlation between Zn and AGEs, and a strong positive correlation between AGEs and the Gly/Zn ratio, suggesting that both Zn and AGEs are biomarkers that could reflect the persistence of hyperglycemia. We identified new surrogate biomarkers useful for the assessment of glycemic control with great potential for the development of preventive and therapeutic strategies for elderly diabetics, based on the evaluation of serum Zn levels.
锌缺乏在许多病理状况中都有报道，如糖尿病，在衰老的生理过程中也存在。同样，糖氧化过程的终产物——晚期糖基化终产物（AGEs）是具有损害性的化合物，大量报告将它们与几种与年龄相关的慢性疾病的发生和发展联系起来。本研究的目的是分析2型糖尿病（T2DM）老年患者的锌状态、糖氧化应激与胰岛素抵抗（IR）之间的关系。一组52名不吸烟的受试者（9名男性和43名女性，年龄65 - 83岁）参与了这项横断面研究：27名T2DM患者和25名明显健康的对照受试者。使用基于终点比色法的商业试剂盒评估血清锌（Zn）水平，并用荧光分析程序评估血清AGEs。进一步计算葡萄糖与锌的比值（Gly/Zn）、胰岛素与锌的比值（Ins/Zn）和胰岛素锌抵抗指数（HOMA - IR/Zn），以研究血清Zn水平、胰腺β细胞分泌功能与AGEs之间的关联。与对照组相比，T2DM患者的血清胰岛素和Zn水平显著更高。我们发现Zn与AGEs之间存在显著的负相关，AGEs与Gly/Zn比值之间存在强正相关，这表明Zn和AGEs都是可以反映高血糖持续存在的生物标志物。基于血清Zn水平的评估，我们确定了有助于评估血糖控制的新替代生物标志物，这些标志物在为老年糖尿病患者制定预防和治疗策略方面具有巨大潜力。
