Department of Gynecology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
Department of Discipline Inspection Commission, China Medical University, Shenyang, Liaoning 110001, P.R. China.
Mol Med Rep. 2021 Dec;24(6). doi: 10.3892/mmr.2021.12496. Epub 2021 Oct 15.
The mechanisms underlying cervical cancer progression have not yet been fully elucidated; thus, further investigations are required. Chaperonin containing TCP1 subunit 3 (CCT3) expression was found to be upregulated in several types of human cancer. However, the roles of CCT3 in cervical cancer remain poorly understood. Thus, the present study aimed to determine the roles of CCT3 in the progression of cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC). For this purpose, the Tumor Immune Estimation Resource and Gene Expression Profiling Interactive Analysis databases were used to analyze the mRNA and protein expression levels of CCT3 in CESC samples. The effects of CCT3 on the proliferation and migration of CESC were determined using various experiments, including proliferation, Transwell and flow cytometric assays. The results revealed that CCT3 expression was significantly upregulated in CESC, which was associated with a poor prognosis. The silencing of CCT3 suppressed CESC cell proliferation, migration and invasiveness . Additionally, CCT3‑knockdown promoted CESC cell apoptosis and cell cycle arrest, and suppressed fibronectin 1 (FN1) protein expression. Furthermore, rescue assays demonstrated that CCT3 promoted CESC proliferation and migration via FN1. In conclusion, the findings of the present study demonstrated that CCT3 is closely associated with the progression of CESC. Thus, CCT3 may be considered a novel, promising biomarker, and a possible therapeutic target for CESC.
宫颈癌进展的机制尚未完全阐明；因此，需要进一步的研究。伴侣蛋白含 TCP1 亚基 3 (CCT3) 的表达在几种类型的人类癌症中上调。然而，CCT3 在宫颈癌中的作用仍知之甚少。因此，本研究旨在确定 CCT3 在宫颈鳞状细胞癌和宫颈内膜腺癌 (CESC) 进展中的作用。为此，使用肿瘤免疫评估资源和基因表达谱分析交互分析数据库分析了 CESC 样本中 CCT3 的 mRNA 和蛋白表达水平。通过增殖、Transwell 和流式细胞术等各种实验确定了 CCT3 对 CESC 增殖和迁移的影响。结果表明，CCT3 在 CESC 中表达明显上调，与预后不良相关。沉默 CCT3 抑制 CESC 细胞增殖、迁移和侵袭。此外，CCT3 敲低促进 CESC 细胞凋亡和细胞周期停滞，并抑制纤连蛋白 1 (FN1) 蛋白表达。此外，挽救实验表明，CCT3 通过 FN1 促进 CESC 的增殖和迁移。综上所述，本研究的结果表明，CCT3 与 CESC 的进展密切相关。因此，CCT3 可能被认为是 CESC 的一种新型有前途的生物标志物和潜在治疗靶点。
