Xu Sheng, Chen Ying, Qi Jingru, Wang Runpeng, Wei Erjun, Wang Qiang, Zhang Yiling, Tang Xudong, Shen Zhongyuan
College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China.
Sericulture Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212100, China.
J Fungi (Basel). 2024 Mar 20;10(3):229. doi: 10.3390/jof10030229.
Chaperonin containing tailless complex polypeptide 1 (CCT) is a molecular chaperone protein that consists of eight completely different subunits and assists in the folding of newly synthesized peptides. The zeta subunit of CCT is a regulatory factor for the folding and assembly of cytoskeletal proteins as individuals or complexes. In this study, the zeta subunit of (NbCCTζ) is identified for the first time. The complete ORF of the gene is 1533 bp in length and encodes a 510 amino acid polypeptide. IFA results indicate that NbCCTζ is colocalized with actin and β-tubulin in the cytoplasm during the proliferative phase and that NbCCTζ is completely colocalized with NbCCTα in the cytoplasm of throughout the entire life cycle. Furthermore, the yeast two-hybrid assay revealed that the NbCCTζ interacts with NbCCTα. The transcriptional level of is significantly downregulated by knocking down the gene, while the transcriptional level of is downregulated after knocking down the gene. These results suggest that NbCCTζ may play a vital role in the proliferation of by coordinating with NbCCTα.
含无尾复合多肽1的伴侣蛋白（CCT）是一种分子伴侣蛋白，由八个完全不同的亚基组成，协助新合成的肽段折叠。CCT的ζ亚基是细胞骨架蛋白作为个体或复合物进行折叠和组装的调节因子。在本研究中，首次鉴定了（NbCCTζ）的ζ亚基。该基因的完整开放阅读框长度为1533 bp，编码一个510个氨基酸的多肽。免疫荧光分析结果表明，在增殖期，NbCCTζ在细胞质中与肌动蛋白和β微管蛋白共定位，并且在整个生命周期中，NbCCTζ在（此处原文缺失相关内容）的细胞质中与NbCCTα完全共定位。此外，酵母双杂交试验表明NbCCTζ与NbCCTα相互作用。通过敲低该基因，（此处原文缺失相关基因名称）的转录水平显著下调，而敲低（此处原文缺失相关基因名称）基因后，（此处原文缺失相关基因名称）的转录水平下调。这些结果表明，NbCCTζ可能通过与NbCCTα协同作用，在（此处原文缺失相关内容）的增殖中发挥重要作用。
