Department of Chemistry and Chemical Biology, Purdue School of Science, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202, United States of America; Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, IN 46202, United States of America.
Biochim Biophys Acta Proteins Proteom. 2022 Jan;1870(1):140732. doi: 10.1016/j.bbapap.2021.140732. Epub 2021 Oct 12.
Native mass spectrometry (MS), the analysis of proteins and protein complexes from solutions that stabilize native solution structures, is a rapidly expanding area. There is strong evidence supporting the retention of proteins' native folds in the absence of solvent under the experimental timescales of MS experiments. Therefore, instrumentation has been developed to use gas-phase native-like protein ions to exploit the speed, sensitivity, and selectivity of mass spectrometry approaches to solve emerging problems in structural biology. This article reviews some of the recent advances and applications in gas-phase instrumentation for structural proteomics.
天然质谱（MS）是对从稳定天然溶液结构的溶液中分离出的蛋白质和蛋白质复合物进行分析，是一个快速发展的领域。有强有力的证据表明，在 MS 实验的实验时间尺度内，蛋白质在缺乏溶剂的情况下仍保持其天然构象。因此，已经开发了仪器来使用气相类似天然的蛋白质离子，以利用质谱方法的速度、灵敏度和选择性来解决结构生物学中的新出现的问题。本文综述了近年来在用于结构蛋白质组学的气相仪器方面的一些进展和应用。
