Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing 210037, China.
Centre Testing International Group Co., Ltd., Suzhou 215134, China.
Bioresour Technol. 2022 Jan;343:126142. doi: 10.1016/j.biortech.2021.126142. Epub 2021 Oct 13.
In this work, effects of sulfomethylated lignins (SLs) prepared from masson pine (SL) and poplar (SL) on enzymatic hydrolysis and cellulase-lignin interaction were comparatively investigated. The results showed that both SL and SL significantly promoted the substrate enzymatic digestibility. The total sugar yield increased from 38.6% to 74.4% and ∼ 100%, respectively at 10 FPU/g-cellulose of cellulase dosage. The protein content in hydrolysate linearly increased with the addition of SL (0 - 1.6 g/g-substrate lignin), which suggested the competitive adsorption of cellulase may occur to substrate lignin and SLs. Further structural analysis of lignin revealed the high S/(V + H) ratio was directly related to the high enzymatic saccharification efficiency. The strong interaction between SL and cellulase decreased the nonproductive adsorption of cellulase onto substrate lignin and increased the accessibility of cellulase to carbohydrate, which was considered to be the key factor for the improvement of substrate enzymatic digestibility.
在这项工作中，比较研究了由马尾松（SL）和杨木（SL）制备的磺甲基化木质素（SL）对酶解和纤维素酶-木质素相互作用的影响。结果表明，SL 和 SL 都显著促进了底物的酶解。在纤维素酶用量为 10 FPU/g-纤维素时，总糖收率分别从 38.6%提高到 74.4%和~100%。水解液中的蛋白质含量随 SL 的添加量（0-1.6 g/g-底物木质素）呈线性增加，这表明纤维素酶可能会与底物木质素和 SL 发生竞争吸附。进一步对木质素结构分析表明，高 S/（V+H）比值与高酶解效率直接相关。SL 与纤维素酶之间的强相互作用降低了纤维素酶对底物木质素的非生产性吸附，增加了纤维素酶对碳水化合物的可及性，这被认为是提高底物酶解效率的关键因素。
