Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China; Joint International Research Lab of Lignocellulosic Functional Materials, International Innovation Center for Forest Chemicals and Materials, Nanjing Forestry University, Nanjing 210037, China.
Bioresour Technol. 2023 Sep;384:129276. doi: 10.1016/j.biortech.2023.129276. Epub 2023 Jun 7.
Sulfonated lignin can significantly enhance the enzymatic hydrolysis of lignocellulose substrates. Lignin is a type of polyphenol, therefore, sulfonated polyphenol, such as tannic acid, is likely to have similar effects. In order to obtain a low-cost and high-efficiency additive to improve enzymatic hydrolysis, sulfomethylated tannic acids (STAs) with different sulfonation degrees were prepared and their impact on enzymatic saccharification of sodium hydroxide-pretreated wheat straw were investigated. Tannic acid strongly inhibited, while STAs strongly promoted the substrate enzymatic digestibility. While adding 0.04 g/g-substrate STA containing 2.4 mmol/g sulfonate group, the glucose yield increased from 60.6% to 97.9% at a low cellulase dosage (5 FPU/g-glucan). The concentration of protein in enzymatic hydrolysate significantly increased with the added STAs, indicating that cellulase preferentially adsorbed with STAs, thereby reducing the amount of cellulase nonproductively anchored on substrate lignin. This result provides a reliable approach for establishing an efficient lignocellulosic enzyme hydrolysis system.
磺化木质素可以显著提高木质纤维素底物的酶解效率。木质素是一种多酚，因此，磺化多酚（如鞣酸）可能具有类似的效果。为了获得一种低成本、高效率的添加剂来改善酶解，我们制备了不同磺化度的磺甲基化鞣酸（STAs），并研究了它们对氢氧化钠预处理小麦秸秆的酶糖化的影响。鞣酸强烈抑制，而 STAs 则强烈促进了底物的酶可消化性。在添加 0.04 g/g-底物含 2.4 mmol/g 磺酸基的 STA 时，在低纤维素酶用量（5 FPU/g-葡聚糖）下，葡萄糖产率从 60.6%提高到 97.9%。随着添加的 STAs，酶解液中蛋白质的浓度显著增加，表明纤维素酶优先与 STAs 吸附，从而减少了非生产性地锚定在底物木质素上的纤维素酶的量。这一结果为建立高效的木质纤维素酶水解体系提供了可靠的方法。
