Polymer Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria 21934, Egypt; Key Laboratory of Science & Technology of Eco-Textile, Ministry of Education, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
Medical Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg EL-Arab City 21934, Alexandria, Egypt.
Int J Biol Macromol. 2021 Dec 15;193(Pt A):293-299. doi: 10.1016/j.ijbiomac.2021.10.039. Epub 2021 Oct 14.
Cancer has become a serious disease threatening human health. To tackle this issue, developing the existing potent anticancer drugs is critical to reducing the time and cost associated with creating a new drug from scratch. Diethyldithiocarbamate (DDC) - an anticancer drug- has received considerable attention due to its selectivity and reactivity. In this study, we prepared a nanofibrous matrix from silk fibroin/polyethylene oxide loaded with diethyldithiocarbamate (DDC@SF/PEO) from an aqueous solution via an electrospinning process. Upon DDC incorporation, the nanofiber's diameter has increased from 450 nm (SF/PEO) to 1202 nm (DDC@SF/PEO) confirming the successful incorporation of DDC. Furthermore, the hydrophobicity of DDC@SF/PEO nanofibrous matrix was improved by turning SF structure from random coil (silk I) to β-sheet (silk II) through ethanol vapor treatment. Biocompatibility of DDC@SF/PEO nanofibrous matrix on human normal cells (Wi-38) showed it was safe and the apoptosis-mediated anticancer activity of DDC was enhanced. Thus, loading DDC on SF/PEO nanofibrous matrix is the key descriptor for enhanced anticancer efficacy of DDC. Considering the all-aqueous and simplistic process, the DDC@SF/PEO nanofibrous matrix could be a promising candidate for cancer treatment applications.
癌症已成为威胁人类健康的严重疾病。为了解决这个问题，开发现有的强效抗癌药物对于减少从头开发新药所需的时间和成本至关重要。二乙基二硫代氨基甲酸盐（DDC）-一种抗癌药物-因其选择性和反应性而受到广泛关注。在这项研究中，我们通过静电纺丝工艺从丝素/聚氧化乙烯的水溶液中制备了负载二乙基二硫代氨基甲酸盐（DDC@SF/PEO）的纳米纤维基质。DDC 掺入后，纳米纤维的直径从 450nm（SF/PEO）增加到 1202nm（DDC@SF/PEO），证实了 DDC 的成功掺入。此外，通过乙醇蒸气处理将 SF 结构从无规卷曲（丝 I）转变为β-折叠（丝 II），提高了 DDC@SF/PEO 纳米纤维基质的疏水性。DDC@SF/PEO 纳米纤维基质对人正常细胞（Wi-38）的生物相容性表明其是安全的，并且 DDC 的凋亡介导的抗癌活性得到增强。因此，将 DDC 负载在 SF/PEO 纳米纤维基质上是增强 DDC 抗癌功效的关键描述符。考虑到全水相和简单的工艺，DDC@SF/PEO 纳米纤维基质可能是癌症治疗应用的有前途的候选物。
