ShaykhoIslami Ali, Ghasemian Mohammadreza, Zardast Mahmoud, Farzad Marjan
Surgical Resident, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Cardiovascular Diseases Research Center, Department of Surgery, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Ann Med Surg (Lond). 2021 Oct 9;71:102928. doi: 10.1016/j.amsu.2021.102928. eCollection 2021 Nov.
Intra-abdominal adhesions are typically found after the most surgical procedures. Normally, most adhesions are asymptomatic; however, few individuals experience postoperative adhesion-related problems such as small bowel obstruction, pelvic pain, infertility, or other complications. We aimed to evaluate the preventive effect of the ascites fluid for postoperative peritoneal adhesions in rat models.
This experimental trial was conducted in Sixty Syrian male rat randomly assigned to six groups of 10 animals each as follows: control (group 1&4); normal saline (group 2&5): 2 mL of normal saline was poured into the peritoneal cavity; and case (group 3&6): 2 mL ascites fluid was poured into the peritoneal cavity. All animals in the six groups underwent laparotomy and measurable serosal injury were created with a standard technique. 10 and 30 days after initial surgery, the rats underwent another laparotomy in groups 1, 2, 3 and 4, 5, 6, respectively to assess macroscopic and microscopic adhesions, which were scored by an examiner who was blind to the animals̕ group assignment. Data analyzed by SPSS version 18, using the kruskal Wallis and Bonferroni-corrected Mann-Whitney U tests. P-values of less than 0.05 were considered significant.
The mean scores of both microscopic and macroscopic adhesion were significantly different between all the groups (P < 0.05). Total macroscopic and microscopic adhesion scores were significantly lower in the ascites fluid treatment than in the control (P = 0.0001) or the normal saline (P < 0.001) group. There was no significant difference between adhesion intensity 10 and 30 days after laparotomy (P > 0.05).
Ascites fluid can decrease the possibility of post-operative intraperitoneal adhesion formation.
腹腔粘连通常在大多数外科手术后出现。通常，大多数粘连是无症状的；然而，少数个体经历与术后粘连相关的问题，如小肠梗阻、盆腔疼痛、不孕或其他并发症。我们旨在评估腹水对大鼠模型术后腹膜粘连的预防作用。
本实验在60只叙利亚雄性大鼠中进行，随机分为6组，每组10只动物，分组如下：对照组（第1组和第4组）；生理盐水组（第2组和第5组）：将2毫升生理盐水注入腹腔；病例组（第3组和第6组）：将2毫升腹水注入腹腔。六组所有动物均接受剖腹手术，并采用标准技术造成可测量的浆膜损伤。初次手术后10天和30天，第1、2、3组和第4、5、6组大鼠分别再次接受剖腹手术，以评估宏观和微观粘连情况，由对动物分组不知情的检查人员进行评分。使用SPSS 18版软件进行数据分析，采用Kruskal Wallis检验和Bonferroni校正的Mann-Whitney U检验。P值小于0.05被认为具有统计学意义。
所有组之间微观和宏观粘连的平均评分均有显著差异（P < 0.05）。腹水治疗组的宏观和微观粘连总评分显著低于对照组（P = 0.0001）或生理盐水组（P < 0.001）。剖腹手术后10天和30天的粘连强度无显著差异（P > 0.05）。
腹水可降低术后腹腔粘连形成的可能性。
