Zhao Wei, Ren Guang-Xu, Qiang Yu, Li Jiaqi, Pu Jinkang, Zhang Yun, Tan Feng, Huang Huicong, Liang Shaohui, Lu Gang
Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China.
Department of Parasitology, Wenzhou Medical University, Wenzhou, China.
Front Vet Sci. 2021 Oct 1;8:714249. doi: 10.3389/fvets.2021.714249. eCollection 2021.
is a microsporidian and zoonotic species. This study investigated the prevalence and distribution of genotypes in farmed masked palm civets using nested PCR, as well as assessed their zoonotic potential by phylogenetic analysis of the ITS region of the rRNA region. Here, we collected 251 fecal specimens from farmed masked palm civets () from the Hainan Island, China. In total, 128 of 251 samples were positive for , with an average infection rate of 51.0%. Seventeen genotypes were identified including 12 known genotypes-HNR-VI ( = 56), SHR1 ( = 45), SHW7 ( = 6), KIN-1 ( = 3), D ( = 3), New1 ( = 3), EbpC ( = 2), CHC5 ( = 1), CHG19 ( = 1), CHN4 ( = 1), EbpA ( = 1), and Henan-III ( = 1)-and five novel genotypes (HNPL-I to HNPL-II; one each). Phylogenetic analysis categorized these genotypes into two groups. Thirteen of them were members of the zoonotic group 1, and the remaining four genotypes were in group 12. This study has shown that the infection rates of in masked palm civets from Hainan were relatively high and provide baseline data to control and prevent microsporidiosis in farm-related communities. Therefore, infections in masked palm civets with zoonotic genotypes D, EbpC, CHN4, EbpA, KIN-1, and Henan-III should be considered potential threats to public health.
是一种微孢子虫和人畜共患病原体。本研究采用巢式PCR调查了养殖果子狸中该病原体的基因型流行情况和分布，并通过对rRNA区域的ITS区域进行系统发育分析评估了它们的人畜共患病潜力。在此，我们从中国海南岛的养殖果子狸（）中收集了251份粪便样本。总共251份样本中有128份对该病原体呈阳性，平均感染率为51.0%。鉴定出17种基因型，包括12种已知基因型——HNR-VI（=56）、SHR1（=45）、SHW7（=6）、KIN-1（=3）、D（=3）、New1（=3）、EbpC（=2）、CHC5（=1）、CHG19（=1）、CHN4（=1）、EbpA（=1）和河南-III（=1）——以及5种新基因型（HNPL-I至HNPL-II；各1种）。系统发育分析将这些基因型分为两组。其中13种是人畜共患病第1组的成员，其余4种基因型在第12组。本研究表明，海南果子狸中该病原体的感染率相对较高，并为控制和预防与养殖相关社区的微孢子虫病提供了基线数据。因此，果子狸感染人畜共患病基因型D、EbpC、CHN4、EbpA、KIN-1和河南-III应被视为对公众健康的潜在威胁。
