Li Juanfeng, Li Dongfang, Zhang Haixia, Wang Rongjun, Lin Zixiang, Zhang Liwei, Cao Yangwenna, Qi Meng
College of Animal Science, Tarim University, Alar, Xinjiang, 843300, China.
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, 450002, China.
Int J Parasitol Parasites Wildl. 2020 Jun 17;12:172-175. doi: 10.1016/j.ijppaw.2020.06.006. eCollection 2020 Aug.
Little is known regarding the genotypes in snakes worldwide. In the present study, a total of 273 fecal samples were collected from pet snakes in Beijing, China. They were then tested for the presence of by PCR amplification of the internal transcribed spacer (ITS) gene. The overall infection rate of was 4.4% (12/273), with the highest infection rate (20%, 1/5) of was found in the Black rat snake (), whereas no positive samples were detected from both Milk (0/22) and Coast garter snakes (0/2). Eight genotypes were identified, including four known genotypes: EbpA (n = 1), EbpC (n = 5), Henan-III (n = 1), and SHR1 (n = 1), and four novel genotypes: CRep-5 (n = 1), CRep-6 (n = 1), CRep-7 (n = 1), and CRep-8 (n = 1). Among them, EbpC (41.7%, 5/12) was the predominant genotype. Phylogenetic analysis showed that seven genotypes belonged to group 1, while genotype SHR1 belonged to group 2. Genotypes EbpA, EbpC, and Henan-III have been previously reported in humans. This suggests that pet snakes are a potential source of zoonotic microsporidiosis transmission in China.
关于全球蛇类的基因型，人们知之甚少。在本研究中，共从中国北京的宠物蛇身上采集了273份粪便样本。然后通过对内部转录间隔区（ITS）基因进行PCR扩增来检测它们是否存在[寄生虫名称未给出]。[寄生虫名称未给出]的总体感染率为4.4%（12/273），在黑鼠蛇（[学名未给出]）中发现了最高感染率（20%，1/5），而在乳蛇（0/22）和海岸束带蛇（0/2）中均未检测到阳性样本。鉴定出了8种基因型，包括4种已知基因型：EbpA（n = 1）、EbpC（n = 5）、河南III型（n = 1）和SHR1（n = 1），以及4种新基因型：CRep - 5（n = 1）、CRep - 6（n = 1）、CRep - 7（n = 1）和CRep - 8（n = 1）。其中，EbpC（41.7%，5/12）是主要基因型。系统发育分析表明，7种基因型属于第1组，而基因型SHR1属于第2组。基因型EbpA、EbpC和河南III型此前已在人类中报道过。这表明宠物蛇是中国动物源性微孢子虫病传播的一个潜在来源。
