Angaramo Santiago, Law Janice C, Maris Alexander Spyros, Schmitz Jonathan Edward, Liu Yuhan, Chen Qingxia, Chomsky Amy
Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
Department of Ophthalmology and Visual Sciences, Vanderbilt Eye Institute, Nashville, Tennessee, USA.
BMJ Open Ophthalmol. 2021 Oct 5;6(1):e000804. doi: 10.1136/bmjophth-2021-000804. eCollection 2021.
The purpose of this study is to investigate the amount of oral flora dispersion towards the ocular surface in relation to various face mask scenarios.
Thirty participants were recruited for this prospective cross-sectional study. Each participant was seated and instructed to hold a blood agar plate perpendicular to the bridge of their nose and facing downward. Participants then partook in three unique face mask scenarios: no face mask, surgical face mask and surgical face mask with tape securing the superior edge. During each scenario, participants were instructed to forcefully exhale for 5 s three times. The primary outcome measure was the number of colony-forming units (CFUs) grown on each face mask scenario-specific plate.
Thirty participants were recruited for the study, and a total of 90 chocolate agar plates were successfully incubated. The proportion of detecting any CFU was 6.67% (95% CI: 0.818% to 22.1%) for no mask scenario, 0% (95% CI: 0% to 11.6%) for mask scenario and 3.33% (95% CI: 0.0844% to 17.2%) for mask-taped scenario. The mean differences in proportion of detecting any CFU were 3.33% (95% CI: 0% to 10%, p=0.309) for no mask versus mask taped, 3.35% (95% CI: 0% to 10%, p=0.307) for mask taped versus mask and 6.68% (95% CI: 0% to 16.7%, p=0.142) for no mask versus mask.
This study showed no difference in bacterial dispersion towards the ocular surface when comparing no face mask, a surgical face mask without tape or a surgical face mask with tape.
本研究旨在调查在不同口罩使用情况下，口腔菌群向眼表扩散的数量。
本前瞻性横断面研究招募了30名参与者。每位参与者就座后，被要求手持一块血琼脂平板，使其垂直于鼻梁并向下。然后，参与者参与三种不同的口罩使用情况：不戴口罩、佩戴外科口罩以及用胶带固定上边缘的外科口罩。在每种情况下，参与者被要求用力呼气3次，每次5秒。主要观察指标是在每种特定口罩使用情况下平板上生长的菌落形成单位（CFU）数量。
本研究招募了30名参与者，共成功培养了90块巧克力琼脂平板。未戴口罩情况下检测到任何CFU的比例为6.67%（95%置信区间：0.818%至22.1%），戴口罩情况下为0%（95%置信区间：0%至11.6%），戴胶带固定口罩情况下为3.33%（95%置信区间：0.0844%至17.2%）。未戴口罩与戴胶带固定口罩相比，检测到任何CFU的比例平均差异为3.33%（95%置信区间：0%至10%，p = 0.309）；戴胶带固定口罩与戴口罩相比，为3.35%（95%置信区间：0%至10%，p = 0.307）；未戴口罩与戴口罩相比，为6.68%（95%置信区间：0%至16.7%，p = 0.142）。
本研究表明，在比较不戴口罩、无胶带的外科口罩和有胶带的外科口罩时，向眼表的细菌扩散没有差异。
