Li Jingjing, Ji Li, Sun Lin, Wei Ningning, Zhu Jingjing, Chen Xiguang, Tai Guihua, Zhou Yifa
Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China; College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China.
Carbohydr Polym. 2012 Jan 4;87(1):551-556. doi: 10.1016/j.carbpol.2011.08.019. Epub 2011 Aug 22.
A water-soluble polysaccharide (HA) was extracted from the Herba Asari root. HA was separated into a starch-like glucan fraction (HA1) and a pectin fraction (HA2) using DEAE-cellulose. HA2 was further fractionated into three pectic polysaccharides, HA2-a, HA2-b and HA2-c, using ion-exchange chromatography. NMR and sugar composition analyses demonstrated that HA2-a is an arabinogalactan (AG) and HA2-b and HA2-c are xylogalacturonans (XGA) with AG domains. Lymphocyte proliferation assays showed that both the neutral polysaccharide and acidic polysaccharide were potent B and T cell stimulators that may have two different modes of action.
从细辛根中提取出一种水溶性多糖（HA）。使用DEAE-纤维素将HA分离为淀粉样葡聚糖级分（HA1）和果胶级分（HA2）。使用离子交换色谱法将HA2进一步分离为三种果胶多糖，即HA2-a、HA2-b和HA2-c。核磁共振（NMR）和糖组成分析表明，HA2-a是一种阿拉伯半乳聚糖（AG），而HA2-b和HA2-c是具有AG结构域的木糖半乳糖醛酸聚糖（XGA）。淋巴细胞增殖试验表明，中性多糖和酸性多糖都是有效的B细胞和T细胞刺激剂，可能有两种不同的作用模式。
