School of Pharmacy, Zunyi Medical University, Zunyi 563006, China.
Department of Laboratory Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, China; School of Laboratory Medicine, Zunyi Medical University, Zunyi 563006, China.
Int J Biol Macromol. 2020 Sep 15;159:455-460. doi: 10.1016/j.ijbiomac.2020.05.051. Epub 2020 May 11.
Pollen has been defined as dietary supplement used to supplement the diet in many countries, but the primary structure and activity of Camellia japonica pollen polysaccharide remain unclear. In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column. Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan. The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached. The results of galectin-3-mediated hemagglutination assay indicated that WCPP-A2a exhibited the strongest inhibitory effect on galectin-3 with MIC value around 0.27 μg/mL. These results suggested the potential use of Camellia japonica pollen polysaccharide as a galectin3 inhibitor in functional foods.
花粉在许多国家被定义为膳食补充剂，用于补充饮食，但山茶花花粉多糖的一级结构和活性仍不清楚。在这项研究中，从山茶花花粉中提取的水溶性多糖（WCPP）通过 DEAE-纤维素柱被分为一个中性部分（WCPP-N）和两个酸性部分（WCPP-A1 和 WCPP-A2），WCPP-A2 进一步通过 Sepharose CL-6B 柱被分为两个均相亚部分（WCPP-A2a 和 WCPP-A2b）。单糖组成结果表明，WCPP-N 可能主要含有淀粉样葡聚糖以及一些阿拉伯半乳聚糖，而 WCPP-A1、WCPP-A2 及其亚部分可能主要由鼠李半乳糖醛酸聚糖 I（RG-I）果胶多糖骨架组成，带有一些不同类型的侧链，包括阿拉伯聚糖、半乳糖聚糖和/或阿拉伯半乳聚糖。通过 NMR 谱分析对 WCPP-A2a 的一级结构分析表明，WCPP-A2a 是一种 RG-I 样果胶多糖，在骨架的 Rha 残基的 O-4 处支化，具有α-（1→3,5）-L-阿拉伯聚糖以及与其相连的 II 型阿拉伯半乳聚糖侧链。半乳糖凝集素-3 介导的血凝抑制试验结果表明，WCPP-A2a 对半乳糖凝集素-3 表现出最强的抑制作用，MIC 值约为 0.27μg/mL。这些结果表明山茶花花粉多糖作为功能食品中半乳糖凝集素 3 抑制剂的潜在用途。
