Shenzhen Key Laboratory of Marine Microbiome Engineering, Shenzhen Key Laboratory of Food Nutrition and Health, Institute for Advanced Study, College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, 518060, China.
College of Biomass Science and Engineering, Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu, 610065, China.
Adv Sci (Weinh). 2024 Jan;11(3):e2308026. doi: 10.1002/advs.202308026. Epub 2023 Nov 28.
Synthetic cell exoskeletons created from abiotic materials have attracted interest in materials science and biotechnology, as they can regulate cell behavior and create new functionalities. Here, a facile strategy is reported to mimic microalgal sporulation with on-demand germination and locomotion via responsive metal-phenolic networks (MPNs). Specifically, MPNs with tunable thickness and composition are deposited on the surface of microalgae cells via one-step coordination, without any loss of cell viability or intrinsic cell photosynthetic properties. The MPN coating keeps the cells in a dormant state, but can be disassembled on-demand in response to environmental pH or chemical stimulus, thereby reviving the microalgae within 1 min. Moreover, the artificial sporulation of microalgae resulted in resistance to environmental stresses (e.g., metal ions and antibiotics) akin to the function of natural sporulation. This strategy can regulate the life cycle of complex cells, providing a synthetic strategy for designing hybrid microorganisms.
由非生物材料制成的合成细胞外壳在材料科学和生物技术领域引起了关注，因为它们可以调节细胞行为并创造新的功能。在这里，报道了一种通过响应性金属-酚网络（MPN）进行按需发芽和运动来模拟微藻孢子形成的简便策略。具体而言，通过一步配位，在微藻细胞表面沉积具有可调厚度和组成的 MPN，而不会损失细胞活力或内在的细胞光合作用特性。MPN 涂层使细胞处于休眠状态，但可以根据环境 pH 值或化学刺激按需分解，从而在 1 分钟内使微藻复活。此外，微藻的人工孢子形成导致对环境胁迫（例如金属离子和抗生素）的抵抗力类似于天然孢子形成的功能。该策略可以调节复杂细胞的生命周期，为设计杂交微生物体提供了一种合成策略。
