Department of Mongolian Osteopath, International Hospital of Mongolian Medicine, Saihan District, Hohhot, Inner Mongolia Autonomous Region, China.
Cardiac Function Department, Cadre Health Care Center, Inner Mongolia Autonomous Region People's Hospital, Saihan District, Hohhot, Inner Mongolia Autonomous Region, China.
Medicine (Baltimore). 2021 Sep 17;100(37):e27183. doi: 10.1097/MD.0000000000027183.
The aim of this study was to identify genes and functional pathways associated with damaged cartilage tissues of knee using microarray analysis.The gene expression profile GSE129147 including including 10 knee cartilage tissues from damaged side and 10 knee nonweight-bearing healthy cartilage was downloaded and bioinformatics analysis was made.A total of 182 differentially-expressed genes including 123 up-regulated and 59 down-regulated genes were identified from the GSE129147 dataset. Gene ontology and pathway enrichment analysis confirmed that extracellular matrix organization, collagen catabolic process, antigen processing and presentation of peptide or polysaccharide antigen, and endocytic vesicle membrane were strongly associated with cartilage injury. Furthermore, 10 hub differentially-expressed genes with a higher connectivity degree in protein-protein interactions network were found such as POSTN, FBN1, LOX, insulin-like growth factor binding proteins3, C3AR1, MMP2, ITGAM, CDKN2A, COL1A1, COL5A1.These hub genes and pathways provide a new perspective for revealing the potential pathological mechanisms and therapy strategy of cartilage injury.
本研究旨在通过微阵列分析鉴定与膝关节损伤软骨组织相关的基因和功能途径。下载基因表达谱 GSE129147，包括 10 个来自损伤侧膝关节和 10 个非负重健康膝关节软骨的基因表达谱，进行生物信息学分析。从 GSE129147 数据集中共鉴定出 182 个差异表达基因，包括 123 个上调基因和 59 个下调基因。基因本体论和通路富集分析证实，细胞外基质组织、胶原分解代谢过程、肽或多糖抗原的抗原加工和呈递以及内吞小泡膜与软骨损伤密切相关。此外，在蛋白质-蛋白质相互作用网络中发现了 10 个具有较高连接度的枢纽差异表达基因，如 POSTN、FBN1、LOX、胰岛素样生长因子结合蛋白 3、C3AR1、MMP2、ITGAM、CDKN2A、COL1A1 和 COL5A1。这些枢纽基因和通路为揭示软骨损伤的潜在病理机制和治疗策略提供了新的视角。
